DI-UMONS : Dépôt institutionnel de l’université de Mons

Recherche transversale
(titres de publication, de périodique et noms de colloque inclus)
2018-11-21 - Colloque/Présentation - poster - Anglais - 1 page(s)

Ronneau Robin , Su Bao-Lian, Wang Li, Gérin Stéphanie, Calvo Sébastien, Weck Klara, Hantson Anne-Lise , Thomas Diane , "High added value metabolites production in photobioreactors (PBR) using microalgae encapsulated in hybrid material (VALOALGUE* project)" in Journées des Jeunes Chercheurs UGéPE_Nord de France-GEPROC 2018, Villeneuve d'Ascq, France, 2018

  • Codes CREF : Biochimie (DI3112), Biotechnologie (DI3800), Chimie des polymères de synthèse (DI131C), Chimie analytique (DI1314), Chimie organique (DI1313), Génie chimique (DI2721), Biologie (DI3100), Chimie (DI1300)
  • Unités de recherche UMONS : Chimie et Biochimie appliquées (F504)
  • Instituts UMONS : Institut de Recherche en Science et Ingénierie des Matériaux (Matériaux), Institut des Biosciences (Biosciences)
  • Centres UMONS : Biosys (BIOSYS)
Texte intégral :

Abstract(s) :

(Anglais) ///Key words: Gaussia luciferase, Chlamydomonas reinhardtii, microalgae, photobioreactor, encapsulation\\\ Currently, several scientific researches threat about culture of microalgae, due to their ability to produce metabolites like lipids or saccharides. Mass culture of microalgae should allow the production of bio-sourced molecules technologically interesting (bioethanol, biofuel,…). But microalgae can also produce, in smaller quantity, high added value metabolites, naturally produced or resulting from a genetic transformation. Chlamydomonas reinhardtii can be considered as a model for this kind of researches. In fact, this microalgae can be modified for the purpose of producing and excreting a selected metabolite like recombinant proteins. However, given the size of microalgae, free cell cultures are not workable to collect molecules in the culture media. The encapsulation of the microalgae in a porous material should be able, at the same time, to hold in microalgae and to allow diffusion of metabolites. The recovery of the metabolites present in the culture medium should therefore be easier. In this research, Chlamydomonas reinhardtii has been modified to produce Gaussia luciferase [1][2], a recombinant protein that has the particularity to emit light in presence of its substrate (coelenterazine). Nowadays, Gaussia luciferase does not present any particular interest, but was selected for its quantification by bioluminescence property. After proving the effectiveness of the concept, some more interesting recombinant proteins will be study. One of the challenges of the project is the choice of the porous material because it must meet several criteria: biocompatibility, good mechanical resistance and allowing diffusion of nutrients and excreted metabolites. Alginate and alginate/silica beads are currently being investigated and especially the biocompatibility and the diffusion of the Gaussia luciferase. ***VALOALGUE is part of the global project “Algae Factory”, funded by ERDD***