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2019-07-06 - Colloque/Présentation - poster - Anglais - page(s)

Delacuvellerie Alice , Wattiez Ruddy , "Alcanivorax borkumensis, a key player for the low-density polyethylene degradation" in FEMS, Glasgow, Ecosse, 2019

  • Codes CREF : Microbiologie et protistologie [bacteriol.,virolog.,mycolog.] (DI3130)
  • Unités de recherche UMONS : Protéomie et Microbiologie (S828)
  • Instituts UMONS : Institut des Biosciences (Biosciences)
Texte intégral :

Abstract(s) :

(Anglais) Background: Most plastics are released to the environment in landfills and end up in the sea, inducing large ecological and health impacts. Nevertheless, plastics could constitute a physical substrate and potential carbon source for microorganisms. Objectives: Highlight new strains of bacteria that could be good candidates for plastic degradation and increase knowledge concerning plastic degradation mechanism. Methods: In an attempt to select bacteria which potentially degrade different type of plastics (PET, PS, LDPE), an enrichment method was used. Bacterial consortia, sampled from marine plastisphere (Mediterranean, Corsica), were grown with a piece of plastic film as the main carbon source and a low percentage of extra carbon (0.05% yeast extract). After the selection and isolation of Alcanivorax borkumensis, its capacity to form biofilm and degrade plastics was monitored by crystal violet and the weight loss methods respectively. Results: Several Genera containing hydrocarbon-degrading bacteria species were enriched on several plastics and species association were observed including Alcanivorax borkumensis specifically selected on LDPE. Results demonstrate clearly that A. borkumensis forms large biofilms on the plastic surface and was also able to degrade LDPE. For the first time, we showed its capacity to degrade a solid structure based to alkane such as petroleum-based plastic LDPE. The mechanism of a partial depolymerization of LDPE – a crucial step for its degradation by Alcanivorax borkumensis is unknown and will be further investigated.