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2014-11-25 - Colloque/Présentation - poster - Anglais - 1 page(s)

Henoumont Céline , Laurent Sophie , Muller Robert , Vander Elst Luce , "DOSY in HR-MAS : a tool to be used with caution" in YBMRS (13ème edition), Spa Sol Cress, Belgique, 2014

  • Codes CREF : Physico-chimie générale (DI1320), Chimie des colloïdes (DI1329)
  • Unités de recherche UMONS : Chimie générale, organique et biomédicale (M108)
  • Instituts UMONS : Institut des Sciences et Technologies de la Santé (Santé), Institut des Biosciences (Biosciences)
  • Centres UMONS : Centre de Recherche en Microscopie et Imagerie Médicale (CMMI)

Abstract(s) :

(Anglais) Our laboratory is developping since several years iron oxide nanoparticles for molecular imaging by MRI. Molecular imaging aims to visualize at an early stage molecular entities which are expressed or overexpressed in pathological conditions. To achieve this, iron oxide nanoparticles are vectorized in order to recognize specifically these molecular targets. The vectorization is performed by grafting at the nanoparticle surface small peptides selected in our laboratory by the phage display technique. Before testing these nanoparticles in vitro and in vivo, it is important to fully characterize them, and particularly it is important to verify the covalent grafting of the small vectors on the nanoparticle surface. A method of choice to achieve it could be NMR, and particularly DOSY. A significant decrease of the diffusion coefficient of the small organic molecules should indeed be observed when they are grafted at the nanoparticle surface. Iron oxide nanoparticles being superparamagnetic, HR-MAS spectroscopy is needed to obtain 1D and 2D spectra of grafted organic molecules, as it has been shown in an article of Polito et al.1 Nevertheless, we will show here that the recording of DOSY spectra in HR-MAS is not so easy and requires to take some precautions, as it was already demonstrated in the literature2. We will also show that it becomes impossible to obtain accurate diffusion coefficients on iron oxide nanoparticles grafted with a test molecule, polyethylene glycol (PEG). 1. Polito, L.; Colombo, M.; Monti, D.; Melato, S.; Caneva, E.; Prosperi, D. J. Am. Chem. Soc. 2008, 130, 12712-12724. 2. Viel, S.; Ziarelli, F.; Pagès, G.; Carrara, C.; Caldarelli, S. J. Magn. Reson. 2008, 190, 113-123.