DI-UMONS : Dépôt institutionnel de l’université de Mons

Recherche transversale
(titres de publication, de périodique et noms de colloque inclus)
2013-09-28 - Colloque/Présentation - poster - Anglais - 1 page(s)

Riaz Maryam , Versaevel Marie , Gabriele Sylvain , "On the mechanism of durotaxis in motile cells" in 5th European Cell Mechanics Meeting, Obergurgl, Austria, 2013

  • Codes CREF : Physico-chimie générale (DI1320), Biophysique (DI3113)
  • Unités de recherche UMONS : Laboratoire Interfaces et Fluides complexes (S885)
  • Instituts UMONS : Institut de Recherche sur les Systèmes Complexes (Complexys), Institut des Biosciences (Biosciences)
  • Centres UMONS : Centre d’Innovation et de Recherche en Matériaux Polymères (CIRMAP)

Abstract(s) :

(Anglais) Cell motility is a fundamental process of many physiological (embryonic development, wound healing,…) and pathological (metastasis development) events. Despite the large interest in durotaxis for stationary cells, few studies have taken into account the impact of ECM stiffness on locomoting cells. To address this issue, we studied the morphology, the directionality and the organization of actin cytoskeleton, adhesions and myosin II in keratocytes migrating on homogeneous 2D substrates over a wide range of stiffnesses, from 1 kPa up to 70 GPa. Our results highlight a close relationship between morphological and migration parameters, suggesting that the substrate stiffness regulates the polarization of the cell as well as its migration behavior. We demonstrate that the physical linkage between the motile cell cytoskeleton and its substrate is required for cell polarization and migration. Taken together, our results are consistent with a physical model in which keratocyte shape and migratory behavior emerge from the self-organization of actin, adhesions, and myosin, and quantitative changes in ECM stiffness impact on these parameters to switch keratocytes among qualitatively distinct migration regimes.