DI-UMONS : Dépôt institutionnel de l’université de Mons

Recherche transversale
(titres de publication, de périodique et noms de colloque inclus)
2016-10-28 - Colloque/Présentation - poster - Anglais - 1 page(s)

Cyriaque Valentine , Jacquiod Samuel, Riber Leise, Abu Al-Soud Waleed, Milani Stefan, Gillan David , Sørensen Søren J., Wattiez Ruddy , "Heavy metal accumulation shaped presence and potential activity of sediment bacteria" in Annual Meeting of the Belgian Society for Microbiology (BSM) , Brussels, Belgium, 2016

  • Codes CREF : Environnement et pollution (DI3840), Microbiologie et protistologie [bacteriol.,virolog.,mycolog.] (DI3130)
  • Unités de recherche UMONS : Protéomie et Microbiologie (S828)
  • Instituts UMONS : Institut des Biosciences (Biosciences)
Texte intégral :

Abstract(s) :

(Anglais) Heavy metal contamination of soils and sediments constitutes a serious issue because of biotoxicity and bioaccumulation. Recent methodological improvement of 16S rRNA profiling has improved analytical accuracy and revealed heavy metals as drivers of microbial community structure. For the present study, metal-contaminated river sediments sampled nearby the MetalEurop foundry (Northern France) were compared to unpolluted sediments collected upstream (Férin). The MetalEurop foundry rejected mainly zinc, copper, cadmium and lead directly in the air and the river during a century. DNA and RNA were extracted from sediments and cDNA was synthetized. DNA and cDNA were submitted to Illumina® MiSeq® sequencing (2x250 bp). Results show that 70% of the bacterial reads (DNA and cDNA) were common between the control and the contaminated sediments. This can be explained by the constant influx of bacteria from the upstream river. However, contaminated sediment harboured a significantly different potentially active community (RNA signal). Moreover, while the amount of extracted DNA was lower in contaminated sediments, the richness and Chao-1 indexes were more elevated when compared to Férin. These results suggest that constant input of bacteria is complemented with metal selection uncovering a broader spectrum of the community. Bacterial tolerance ranges were characterized using the macro-ecological concept of Functional Response Group by clustering OTUs based on their cDNA/DNA abundance patterns, revealing 6 groups that highlight the taxonomic richness of the contaminated sediments.

Identifiants :
  • FNRS : FC 7231