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2014-06-22 - Colloque/Présentation - poster - Anglais - 1 page(s)

Helvenstein Maxime, Stanicki Dimitri , Blankert Bertrand , "In vitro evaluation of the interaction of iron oxide nanoparticles with the HepaRG cells" in Drug Analysis, Liège, Belgique, 2014

  • Codes CREF : Chimie analytique (DI1314)
  • Unités de recherche UMONS : Chimie générale, organique et biomédicale (M108), Analyse pharmaceutique (M130)
  • Instituts UMONS : Institut des Sciences et Technologies de la Santé (Santé)

Abstract(s) :

(Anglais) Iron oxide nanoparticles are principally used to label cells for magnetic resonance imaging (MRI) tracking. Nowadays, magnetic labeling give rise to promising new opportunities for tissue engineering by controlling and manipulating cells with an external magnetic field to influence the organization and the migration of them [1-2]. Here we describe a non-specific labeling of metabolic competent HepaRG cells with anionic iron oxide nanoparticles. HepaRG cells are principally used to perform drug metabolism and toxicity studies of xenobiotics because they express and maintain a large panel of phase I and phase II enzymes [3-5]. An interaction was observed between nanoparticles and cells and these can be easily attracted with magnet. No cytotoxicity (MTT assay) was found on cells after an incubation time of 24 hours with nanoparticles at different concentrations. HepaRG metabolization activity using diclofenac as reference substance was also evaluated and seems to be affected after incubation with nanoparticles at 2mM in iron. Based on these results, new strategies could be considered in 3D culture or in transplantation (MRI detection) research domains with HepaRG cell line. Even if further investigations should be considered to assess the possible impact on metabolization activity.