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2009-04-01 - Colloque/Présentation - poster - Anglais - 1 page(s)

Sclavons Coralie, Burtea Carmen , Laurent Sophie , Toubeau Gérard, Vander Elst Luce , Muller Robert , "MRI targeting of apoptotic cells in injured areas of brain in a mouse model of Parkinson’s disease" in Biomedica, Liège, Belgique, 2009

  • Codes CREF : Histologie (DI3212), Sciences biomédicales (DI3200), Neuropathologie (DI332C), Chimie organique (DI1313), Chimie des colloïdes (DI1329), Imagerie médicale, radiologie, tomographie (DI3243)
  • Unités de recherche UMONS : Chimie générale, organique et biomédicale (M108), Histologie (M118)
  • Instituts UMONS : Institut des Sciences et Technologies de la Santé (Santé), Institut des Biosciences (Biosciences)
  • Centres UMONS : Centre de Recherche en Microscopie et Imagerie Médicale (CMMI)

Abstract(s) :

(Anglais) Introduction Parkinson’s disease (PD) is a neurodegenerative disorder characterized by a massive loss of dopaminergic neurons located in the basal ganglia, both by apoptosis and by neuroinflammation. In order to facilitate early diagnosis of this disease, a peptide specific to apoptotic cells identified by phage display [1] was grafted to a magnetic reporter and used to image injured areas in PD by MRI. Methodology The peptide (PPS), specific to phosphatidylserine exposed on the outer leaflet of cell membrane during apoptosis, has been conjugated to ultra small particles of iron oxide (USPIO-PPS) and injected to mice treated with MPTP (1-methyl 4-phenyl 1,2,3,6-tetrahydropyridine), which is a neurotoxin inducing PD. The mice were then analyzed by MRI on a Bruker AVANCE-200 spectrometer working at 4.7T and equipped with a vertical magnet and a micro-imaging system. Apoptosis and targeting by contrast agents were confirmed on dopaminergic neuron cultures. Results The MR images acquired on MPTP mice injected with USPIO-PPS showed that the targeted areas in brain correspond to the injured region in parkinsonian mice; dopaminergic neurons were immunostained after MRI sessions with anti-tyrosine hydroxylase antibody. The same mice injected with control non-vectorized agent (USPIO) did not reveal any particular targeting in the injured areas of the brain . An apoptotic activity (caspase-3) was detected in cultures of dopaminergic neurons after exposure to MPP+ (MPTP substrate), while USPIO-PPS uptake was confirmed both by MRI and by measuring iron concentration in cell pellets. Conclusion Affected areas in PD can be imaged in MPTP mice by targeting apoptotic cells with USPIO vectorized by a phosphatidylserine-specific peptide. References [1] Burtea C (2007). Molecular imaging of atherosclerosis: Research of phage display-selected peptides with specific affinity for biomolecules overexpressed in vulnerable atherosclerotic plaques. UMH, Mons, Belgium, Ph.D. in biomedical sciences.


Mots-clés :
  • (Anglais) MRI contrast agents
  • (Anglais) peptides
  • (Anglais) Parkinson's disease
  • (Anglais) functionalized iron oxide nanoparticles
  • (Anglais) apoptosis