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2019-05-11 - Colloque/Présentation - poster - Anglais - 1 page(s)

Versaevel Marie , Alaimo Laura , Luciano Marine , Mohammed Danahe, Vercruysse Eléonore , Gabriele Sylvain , "Collective migration during a gap closure in a two-dimensional haptotactic model" in Approche quantitative du vivant, Hyères, France, 2019

  • Codes CREF : Physico-chimie générale (DI1320)
  • Unités de recherche UMONS : Laboratoire Interfaces et Fluides complexes (S885)
  • Instituts UMONS : Institut des Biosciences (Biosciences)
  • Centres UMONS : Centre d’Innovation et de Recherche en Matériaux Polymères (CIRMAP)

Abstract(s) :

(Anglais) The ability of cells to respond to substrate-bound protein gradients is crucial for many physiological processes such as immune response, neurogenesis1 and cancer cell motility2. However, the difficulty to produce well-controlled protein gradients limits our understanding of collective cell migration in response to haptotaxis. To address this issue, we benefit from the recent development of the PRIMO photopatterning technique3. We microprinted circular or linear gradients of fibronectin on two-dimensional (2D) cell culture substrates. We observed that epithelial cells spread preferentially on zones of higher fibronectin densities, creating a gap within the epithelium over circular or linear zones of lower fibronectin densities. Using time- lapse experiments, we demonstrate that gap closures in a 2D haptotaxis model require a significant increase of leader cell4 area over the zone of lower protein density. Increasing cell spreading during closure enables to close the gap with similar dynamics than on homogeneous FN coatings but leads to regions of lower cell densities over the gaps. Interestingly, our findings show that proliferation increases rapidly after closure to restore the tissue density and strengthen cadherin junctions in matured tissues.