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2021-04-29 - Article/Dans un journal avec peer-review - Anglais - 18 page(s)

Delcourt Manon , Delsinne Virginie , Colet Jean-Marie , Decleves Anne-Emilie , Tagliatti Vanessa , "Investigation of Mitochondrial Adaptations to Modulation of Carbohydrate Supply during Adipogenesis of 3T3-L1 Cells by Targeted H-NMR Spectroscopy" in Biomolecules, 11, 5, 10.3390/biom11050662.

  • Edition : Multidisciplinary Digital Publishing Institute (MDPI) (Switzerland)
  • Codes CREF : Diabétologie (DI3373)
  • Unités de recherche UMONS : Biochimie métabolique et moléculaire (M122)
  • Instituts UMONS : Institut des Sciences et Technologies de la Santé (Santé)
Texte intégral :

Abstract(s) :

(Anglais) Background: White adipose tissue (WAT) is a dynamic and plastic tissue showinghigh sensitivity to carbohydrate supply. In such a context, the WAT may accordingly modulate itsmitochondrial metabolic activity. We previously demonstrated that a partial replacement of glucoseby galactose in a culture medium of 3T3-L1 cells leads to a poorer adipogenic yield and improvedglobal mitochondrial health. In the present study, we investigate key mitochondrial metabolic actorsreflecting mitochondrial adaptation in response to different carbohydrate supplies. (2) Methods:The metabolome of 3T3-L1 cells was investigated during the differentiation process using differentglucose/galactose ratios and by a targeted approach using1H-NMR (Proton nuclear magneticresonance) spectroscopy; (3) Results: Our findings indicate a reduction of adipogenic and metabolicoverload markers under the low glucose/galactose condition. In addition, a remodeling of themitochondrial function triggers the secretion of metabolites with signaling and systemic energeticalhomeostasis functions. Finally, this study also sheds light on a new way to consider the mitochondrialmetabolic function by considering noncarbohydrates related pathways reflecting both healthiercellular and mitochondrial adaptation mechanisms; (4) Conclusions: Different carbohydrates suppliesinduce deep mitochondrial metabolic and function adaptations leading to overall adipocytes functionand profile remodeling during the adipogenesis.