DI-UMONS : Dépôt institutionnel de l’université de Mons

Recherche transversale
Rechercher
(titres de publication, de périodique et noms de colloque inclus)
2013-01-29 - Colloque/Présentation - poster - Anglais - 1 page(s)

Paci Paula , Ris Laurence , Godaux Emile, "Expression of Arc protein in hippocampal organotypic cultures" in Journée scientifique des doctorants en Sciences Biomédicales et Pharmaceutiques: "New tools and strategies in identification of pathology-related biomarkers", UMONS, Belgique, 2013

  • Codes CREF : Sciences biomédicales (DI3200)
  • Unités de recherche UMONS : Neurosciences (M119)
  • Instituts UMONS : Institut des Sciences et Technologies de la Santé (Santé)

Abstract(s) :

(Anglais) Memory is encoded in our brain by modifications of synaptic efficacy between neurons. Synaptic plasticity is the ability of synapses to modify their efficiency after previous neuronal activity. More precisely, we are interested in one kind of synaptic plasticity which consists in a persistent increase of the synaptic transmission, called Long-Term Potentiation (LTP). Arc protein is implicated in memory and learning mechanisms. In a basal state, Arc is not expressed in the hippocampus. However, it is induced during LTP. mRNA of Arc is transported to activated synapses, where it will be locally translated, and finally Arc protein will be localized in dendritic spines (small varicosities on dendrites). To better understand the LTP mechanisms, expression and localization of Arc protein are studied in the hippocampus. We use an in vitro model, hippocampal organotypic slices which have the advantage of preserving the architectural organization of the hippocampus on the long term. These organotypic slices are transfected using the biolistic transfection method. This technique consists in delivering gold particles coated with DNA, by helium pressure. In a first experiment, we induced chemical LTP in slices and confirmed the increase of Arc expression by Western blotting. Thanks to biolistic transfection, we were able to visualise dendritic spines using EGFP. In the future, we will transfect an Arc expression vector in order to follow its localization in dendritic spines before and after LTP induction.