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2013-01-29 - Article/Dans un journal avec peer-review - Anglais - 7 page(s)

Grevesse Thomas , Versaevel Marie , Circelli Géraldine , Desprez Sylvain , Gabriele Sylvain , "A simple route to functionalize polyacrylamide hydrogels for the independent tuning of mechanotransduction cues" in Lab on a Chip - Miniaturisation for Chemistry & Biology, 13, 777-780

  • Edition : Royal Society of Chemistry
  • Codes CREF : Physico-chimie générale (DI1320), Chimie des surfaces et des interfaces (DI1327), Biophysique (DI3113)
  • Unités de recherche UMONS : Laboratoire Interfaces et Fluides complexes (S885)
  • Instituts UMONS : Institut de Recherche sur les Systèmes Complexes (Complexys), Institut des Biosciences (Biosciences)
  • Centres UMONS : Centre d’Innovation et de Recherche en Matériaux Polymères (CIRMAP)
Texte intégral :

Abstract(s) :

(Anglais) Physico-chemical and biochemical factors in the local cellular microenvironment are known to impact on multiple aspects of cell behaviour through specific signal pathways. These mechanotransduction cues can couple each other to regulate cell fate, and it remains unclear whether mechanotransduction in different contexts shares common mechanisms. Undoubtedly, a challenge will involve the further characterization of such cooperative mechanisms, as well as clearly defining the individual role of each mechanical and biochemical parameter. To control these mechanotransduction cues in an independent manner, we developed a simple and efficient strategy to immobilize any desired nature of proteins on polyacrylamide hydrogels and independently control various parameters of the cell microenvironment, such as matrix stiffness, cell-binding ligand density and confined adhesiveness. This novel platform is validated by conducting single-cell experiments and opens a broad avenue for studying complex interplays involved in mechanotransduction with a facile and versatile approach.

Identifiants :
  • FNRS : TIRF Microscopy 1.5013.11
  • FNRS : Nanomotility FRFC 2.4622.11

Mots-clés :
  • (Anglais) Hydrogels
  • (Anglais) microengineering
  • (Anglais) mechanotransduction