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2022-01-20 - Colloque/Présentation - poster - Anglais - 1 page(s)

Versaevel Marie , Luciano Marine , Gabriele Sylvain , "Curvature-induced epithelium reorganization within soft microniches" in Quantitative Approaches to Living Systems Days, Paris, France, 2022

  • Codes CREF : Physico-chimie générale (DI1320), Biophysique (DI3113)
  • Unités de recherche UMONS : Laboratoire Interfaces et Fluides complexes (S885)
  • Instituts UMONS : Institut des Biosciences (Biosciences)
  • Centres UMONS : Centre d’Innovation et de Recherche en Matériaux Polymères (CIRMAP)

Abstract(s) :

(Anglais) Conventional 2D cell culture techniques have provided fundamental insights into key biochemical and biophysical mechanisms. However, they do not fully capture the physical and chemical properties of the native cell microenvironment. Indeed, many tissues and organs present complex 3D environments with specific topographies, stiffness and biochemical patterns. These biochemical and mechanical properties of the cellular environment are related to the physiological function of the cells and tissues. The development of innovative cell culture substrates that reproduce the complexity of the 3D cell microenvironment is therefore essential for physiologically-relevant in vitro studies and testing. Here, we present an easy and robust method to produce hydrogel microniches in a wide range of stiffness, covering the whole physiological range (from 1 kPa to 250 kPa). We use the Irgacure 2959 photoinitiator to photopolymerize hydroxy-polyacrylamide (hydroxy-PAAm) hydrogels with ultraviolet illumination. By controlling spatially the UV insulation, we produce soft 3D topographies with varying geometries with a micron-scale resolution. We formed 3D soft hemispheric and bowl-shaped microniches by insulating the hydrogel through an optical photomask with circular patterns. In this work, these bowl-shaped microniches are used to study how matrix curvature impacts cell fate. We study epithelial tissues cultured within bowl-shaped microniches of different diameters and depth, to show that substrate curvature alters tissue morphology that results in nuclear deformation and orientation. This reorganization of cells within the tissue at the periphery and inside the curved microniches affects the cell migration pattern and generates numerous cellular exchanges between the niche and its surrounding environment. We envision that our 3D microniches will have potential applications in understanding how multicellular architectures organize and adapt to well-defined 3D structures.