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2014-12-08 - Article/Dans un journal avec peer-review - Anglais - 6 page(s)

Versaevel Marie , Braquenier Jean-Baptiste, Riaz Maryam, Lantoine Joséphine, Gabriele Sylvain , "Super-resolution microscopy reveals LINC complex recruitment at nuclear indentation sites" in Scientific Reports, 4, 7362

  • Edition : Nature Publishing Group, London (United Kingdom)
  • Codes CREF : Physico-chimie générale (DI1320), Biophysique (DI3113)
  • Unités de recherche UMONS : Laboratoire Interfaces et Fluides complexes (S885)
  • Instituts UMONS : Institut de Recherche en Science et Ingénierie des Matériaux (Matériaux), Institut des Biosciences (Biosciences)
  • Centres UMONS : Centre d’Innovation et de Recherche en Matériaux Polymères (CIRMAP)
Texte intégral :

Abstract(s) :

(Anglais) Increasing evidences show that the actin cytoskeleton is a key parameter of the nuclear remodeling process in response to the modifications of cellular morphology. However, detailed information on the interaction between the actin cytoskeleton and the nuclear lamina was still lacking. We addressed this question by constraining endothelial cells on rectangular fibronectin-coated micropatterns and then using Structured Illumination Microscopy (SIM) to observe the interactions between actin stress fibers, nuclear lamina and LINC complexes at a super-resolution scale. Our results show that tension in apical actin stress fibers leads to deep nuclear indentations that significantly deform the nuclear lamina. Interestingly, indented nuclear zones are characterized by a local enrichment of LINC complexes, which anchor apical actin fibers to the nuclear lamina. Moreover, our findings indicate that nuclear indentations induce the formation of segregated domains of condensed chromatin. However, nuclear indentations and condensed chromatin domains are not irreversible processes and both can relax in absence of tension in apical actin stress fibers.

Identifiants :
  • DOI : 10.1038/srep07362
  • FNRS : Grants “TIRF Microscopy” n°1.5013.11F.
  • FNRS : Grants “Nanomotility” FRFC n°2.4622.11