DI-UMONS : Dépôt institutionnel de l’université de Mons

Recherche transversale
(titres de publication, de périodique et noms de colloque inclus)
2019-10-10 - Colloque/Présentation - communication orale - Anglais - page(s)

Delacuvellerie Alice , Cyriaque Valentine , Gobert Sylvie, Benali Samira , Wattiez Ruddy , "Alcanivorax borkumensis, a key player for the low-density polyethylene degradation" in GCNPM, Riga, Lettonie, 2019

  • Codes CREF : Microbiologie et protistologie [bacteriol.,virolog.,mycolog.] (DI3130)
  • Unités de recherche UMONS : Protéomie et Microbiologie (S828)
  • Instituts UMONS : Institut des Biosciences (Biosciences)
Texte intégral :

Abstract(s) :

(Anglais) Most plastics are released to the environment in landfills and end up in the sea, inducing large ecological and health impacts. Nevertheless, plastics could constitute a physical substrate and potential carbon source for microorganisms. In this study, in an attempt to select bacteria which potentially degrade different type of plastics (PolyEthylene Terephthalate (PET), PolyStyrene (PS), Low Density PolyEthylene (LDPE), an enrichment method was used. Bacterial consortia, sampled from marine plastisphere (Mediterranean, Corsica), were grown with a piece of plastic film as the main carbon source and a low percentage of extra carbon (0.05% yeast extract). Several Genera containing hydrocarbon-degrading bacteria species were enriched on several plastics including Alcanivorax borkumensis specifically selected on LDPE. After the isolation of Alcanivorax borkumensis, its capacity to form biofilm and degrade plastics was monitored by crystal violet and the weight loss methods respectively. Results demonstrate clearly that A. borkumensis forms large biofilms on the plastic surface (Fig.1A) and especially on LDPE films. It seems that this bacterim is able to degrade LDPE (Fig.1B). For the first time, we showed its capacity to degrade a solid structure based to alkane such as petroleum-based plastic LDPE. The mechanism of a partial depolymerization of LDPE – a crucial step for its degradation by Alcanivorax borkumensis - is unknown and will be further investigated.