DI-UMONS : Dépôt institutionnel de l’université de Mons

Recherche transversale
(titres de publication, de périodique et noms de colloque inclus)
2016-06-12 - Article/Dans un journal avec peer-review - Anglais - 9 page(s)

Riaz Maryam, Versaevel Marie , Mohammed Danahe, Glinel Karine, Gabriele Sylvain , "Persistence of fan-shaped keratocytes is a matrix-rigidity-dependent process that require alpha5beta1 integrin engagement" in Scientific Reports, 6, 34141

  • Edition : Nature Publishing Group, London (United Kingdom)
  • Codes CREF : Physico-chimie générale (DI1320), Biophysique (DI3113)
  • Unités de recherche UMONS : Laboratoire Interfaces et Fluides complexes (S885)
  • Instituts UMONS : Institut de Recherche en Science et Ingénierie des Matériaux (Matériaux), Institut des Biosciences (Biosciences)
Texte intégral :

Abstract(s) :

(Anglais) Growing evidence suggests that cell migration towards stiffer zones of the extracellular matrix (ECM) via a process called durotaxis contributes to various physiological processes, including wound healing, development and cancer metastasis. However, many aspects of the rigidity-sensing mechanism of motile cells remain elusive. Here we show that the matrix stiffness dictates the level of polarization and directionality of motile keratocytes over seven orders of magnitude in rigidity, while keeping constant their spreading area. Soft substrates lead to nascent adhesions, rounded cell shape and promoted low persistent migration, whereas rigid substrates allowed formation of mature focal adhesions, fan-shaped cells and efficient migratory behaviours. Our results provide compelling evidence that the adaptation of the migratory behaviour to matrix stiffness emerges from the self-organization of actin, myosin and cell-substrate adhesions through an α5β1 dependent rigidity-sensing mechanism.

Identifiants :
  • FNRS : Grants ‘‘Nanomotility’’ FRFC nu2.4622.11
  • FNRS : Grants ‘‘‘TIRF Microscopy’’ nu1.5013.11F.