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2010-03-24 - Article/Dans un journal avec peer-review - Anglais - 8 page(s)

Gabriele Sylvain , Versaevel Marie , Preira Pascal, Theodoly Olivier, "A simple microfluidic method to select, isolate and manipulate single-cells in mechanical and biomechanical assays" in Lab on a Chip - Miniaturisation for Chemistry and Biology, 10, 1459-1467

  • Edition : Royal Society of Chemistry (United Kingdom)
  • Codes CREF : Physico-chimie générale (DI1320), Mécanique des fluides (DI1244), Biophysique (DI3113)
  • Unités de recherche UMONS : Laboratoire Interfaces et Fluides complexes (S885)
  • Instituts UMONS : Institut de Recherche sur les Systèmes Complexes (Complexys), Institut des Biosciences (Biosciences)
  • Centres UMONS : Centre d’Innovation et de Recherche en Matériaux Polymères (CIRMAP)
Texte intégral :

Abstract(s) :

(Anglais) This article describes a simple and low-tech microfluidic method for single-cell experimentation, which permits cell selection without stress, cell manipulation with fine control, and passive self- exclusion of all undesired super-micronic particles. The method requires only conventional soft lithography microfabrication techniques and is applicable to any microfluidic single-cell circuitry. The principle relies on a bypass plugged in parallel with a single-cell assay device and collecting 97% of the flow rate. Cell selection into the single cell device is performed by moving the cell of interest back and forth in the vicinity of the junction between the bypass and the analysis circuitry. Cell navigation is finely controlled by hydrostatic pressure via centimetre-scale actuation of external macroscopic reservoirs connected to the device. We provide successful examples of biomechanical and biochemical assays on living human leukocytes passing through 4 mm wide capillaries. The blebbing process dynamics are monitored by conventional 24 fps videomicroscopy and subcellular cytoskeleton organization is imaged by on-chip immunostaining.

Identifiants :
  • DOI : 10.1039/C002257H