DI-UMONS : Dépôt institutionnel de l’université de Mons

Recherche transversale
Rechercher
(titres de publication, de périodique et noms de colloque inclus)
2019-11-29 - Colloque/Présentation - poster - Anglais - 1 page(s)

Wells Mathilde , Hambye Stéphanie , Blankert Bertrand , "Fractionation process for the isolation of antiplasmodial compounds from the venom of Rhinella marina and Bufo bufo" in Bioforum, Liège, Belgique, 2019

  • Codes CREF : Analyse et contrôle pharmaceutique (DI3450), Sciences pharmaceutiques (DI3400), Techniques séparatives (DI2729)
  • Unités de recherche UMONS : Analyse pharmaceutique (M130)
  • Instituts UMONS : Institut des Sciences et Technologies de la Santé (Santé)

Abstract(s) :

(Anglais) Malaria remains a major concern for health organizations around the world. In 2017, the World Health Organization reported more than 219 million cases and 435.000 deaths. With 87 countries affected, more than 3 billion people are at risk of infection. The emergence and transmission of resistances to most antimalarial drugs are a real worry. Thus, the need for new therapeutic candidates represents an absolute necessity. In more recent years, animal venoms have sparked a growing interest in the scientific community. In fact, toad venoms constitute a rich source of compounds, namely bufadienolides, with many potential therapeutic activities. The objective of this on-going project is to develop a bio-guided fractionation process and the subsequent discovery of new drug candidates against malaria from toad venom. Up to now, two species have been studied: Rhinella marina and Bufo bufo. The extraction process from the air-dried gland secretions of the Bufo toads consists of an ultrasonication-assisted solvent extraction. Two solvents have been tested: methanol and acetonitrile. The venom composition is subject to variabilities between batches depending on the animal’s habitat and its diet. After each extraction, the raw extracts are analyzed by TLC and LC-MS to have an overview of the compounds present in the sample. Flash chromatography is considered as a first approach to obtain rough fractions that will also be analyzed by TLC and LC-MS and then biologically studied. Flash chromatography offers a fast and simple separation process that can be applied to complex natural products. The following step will consist in producing subfractions of the fractions displaying interesting therapeutic properties. Each raw extract and the subsequently obtained fractions are tested for their antiplasmodial activity. The samples that display antiplasmodial activities will be structurally characterized and studied (MS, NMR, etc.).


Mots-clés :
  • (Anglais) venom
  • (Anglais) Malaria
  • (Anglais) toad