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2016-05-27 - Colloque/Présentation - poster - Anglais - 1 page(s)

Valentini Xavier, Deneufbourg Pauline, Paci Paula, Ris Laurence , Frau Annica , Rugira Uwamahoro Pascaline, Nonclercq Denis , "Neurotoxicity caused by the exposition to tiO2 nanoparticles in vitro and in vivo" in Spring meeting of the BSCDB, Leuven, Belgique, 2016

  • Codes CREF : Histologie (DI3212)
  • Unités de recherche UMONS : Histologie (M118)
  • Instituts UMONS : Institut des Sciences et Technologies de la Santé (Santé)

Abstract(s) :

(Anglais) Currently, nanoparticles (NPs) of titanium dioxide (TiO2) are produced abundantly. TiO2 NPs are present in some food products, in paints, cosmetics, toothpastes. The toxicity of TiO2 NPs on the central nervous system has been poorly investigated until now. The aim of this study was to evaluate the toxicity of TiO2 NPs on the central nervous system in vitro and in vivo. In cell cultures derived from embryonic rat, a significant decrease of neuroblasts was observed after 24, 48, 72 and 96h of incubation with TiO2 NPs. This phenomenon resulted from an inhibition of neuroblast proliferation and an increase of apoptosis. In the same time, an astrocytic reaction characterized by an increase of proliferation was observed. The activation of microglial cells was highlighted by immunofluorescence showing the hypertrophy of these cells. The phagocytosis of TiO2 NPs by microgliocytes was also observed. In vivo, after intraperitoneal injection, the TiO2 NPs reached the brain through the blood brain barrier and the nanoparticles promoted various histological injuries such as cellular lysis, neuronal apoptosis and inflammation. An oxidative stress was also detected by immunohistochemistry in hippocampus, cerebellum and in subependymal area. Our study demonstrated the toxic effect of TiO2 NPs on rat brain and neuronal cells.