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2019-01-29 - Article/Dans un journal avec peer-review - Anglais - 5 page(s)

Roch Alain, Prodéo J., Pierart Corinne , Muller Robert , Duez Pierre , "The paramagnetic properties of malaria pigment, hemozoin, yield clues to a low-cost system for its trapping and determination" in Talanta, 197, 553-557, https://doi.org/10.1016/j.talanta.2019.01.090

  • Edition : Elsevier (Netherlands)
  • Codes CREF : Anamnèse - diagnostic [biologie clinique] (DI3316), Chimie analytique (DI1314), Sciences pharmaceutiques (DI3400)
  • Unités de recherche UMONS : Chimie thérapeutique et Pharmacognosie (M136)
  • Instituts UMONS : Institut des Sciences et Technologies de la Santé (Santé)
Texte intégral :

Abstract(s) :

(Anglais) The determination of the hemozoin bound by a gradient magnetic field is investigated as a possible basis for a new method to determine Plasmodium parasitemia. In a manual trapping column system, two types of magnetic filling have been tested to produce field gradients: nickel-plated steel wires, wrapped around a steel core, and superparamagnetic microbeads. The latter system allows an efficient trapping (> 80 %) of -hematin (a synthetic pigment with properties analogous to those of hemozoin). Tests with a Plasmodium falciparum 3D7 culture indicate that hemozoin is similarly trapped. Off-line optical spectroscopy measurements present limited sensitivity, nevertheless allowing the detection of parasitemia in the range of 0.02 % (1000 parasites/µL). Further work needs to be undertaken to reduce this threshold to a practical detectability level. Based on these data, a magneto-chromatographic on-line system with reduced dead volumes is proposed as a possible low-cost instrumental malaria diagnosis system.