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2016-09-05 - Colloque/Présentation - poster - Anglais - 1 page(s)

Bayon-Vicente Guillaume , Deschoenmaeker Frédéric, Sachdeva Neha , Leroy Baptiste , Raffestin Stephanie, Paille Christel, Lasseur Christophe, Wattiez Ruddy , "Functional characterisation of N metabolism within Arthrospira sp. PCC 8005, and its application for the MELiSSA loop" in PRODEX 30th anniversary, Noordwijk, The Netherland, 2016

  • Codes CREF : Biologie (DI3100)
  • Unités de recherche UMONS : Protéomie et Microbiologie (S828)
  • Instituts UMONS : Institut des Biosciences (Biosciences)
Texte intégral :

Abstract(s) :

(Anglais) After decades of researche Humans have succeeded in space traveling. However, some space areas remain unattainable. In this context, the European Space Agency (ESA) has developed the MELiSSA loop in order to proceed to long duration spaceflight by recycling wastes in oxygen, food and water. Arthrospira sp. PCC 8005 has been selected to settle in the fourth compartment (IVa) to consume nitrogen and carbon dioxide yielding biomass and oxygen. Regarding the importance of its role, it is of particular importance to study the impact of different nitrogen sources on its metabolism. Our study first reveals some toxicity phenomena of ammonium ions on growth. In contrast, in a certain range of concentration, urea seems to be suitable for the growth of Arthrospira sp. PCC 8005. Transcriptomic and proteomic analyses indicate repression of nitrate assimilation in presence of NH4+ but also in presence of urea. Finally, the possibility to steer urine from the crew compartment has been investigated by analysing the effect of synthetic urine on the growth and pigment content. Our data point out a negative effect of this medium on growth and pigment content. However, further experiments are required to deeply understand this phenomenon.


Mots-clés :
  • (Anglais) Space research
  • (Anglais) Arthrospira
  • (Anglais) Proteomics
  • (Anglais) Transcriptomics