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2019-03-05 - Colloque/Présentation - poster - Anglais - page(s)

Leveque Mathieu , Hambye Stéphanie , Blankert Bertrand , "Characterization of the binding constant between oligonucleotides and small molecules in the context of Steinert’s disease by affinity capillary electrophoresis" in Mardi des Chercheurs 2019 (MdC2019), Mons, Belgique, 2019

  • Codes CREF : Analyse et contrôle pharmaceutique (DI3450), Chimie analytique (DI1314), Sciences pharmaceutiques (DI3400)
  • Unités de recherche UMONS : Analyse pharmaceutique (M130)
  • Instituts UMONS : Institut des Sciences et Technologies de la Santé (Santé)

Abstract(s) :

(Anglais) Myotonic dystrophy type 1, also named Steinert’s disease, is a genetic pathology characterized by trinucleotides (CTG) repeat expansions. These mutations are associated with an apparition of muscle disorders that affect many systems (muscular, endocrine, central nervous, …). The objective of this project is to determine molecules that could have a sufficient affinity for these mutated nucleotide sequences in order to suppress DNA/RNA sequences causing the pathology. Firstly, DNA synthesis and plasmid extraction protocols will be developed and optimized by bacterial amplification. The next step will be to quantify the binding constant between oligonucleotides and molecules by affinity capillary electrophoresis.