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2016-08-01 - Colloque/Présentation - communication orale - Anglais - 15 page(s)

Delroisse Jérôme , Tian Shi, Weigang Cai, Semmens Dean, Adeiye Odekunle Esther, Alfonso Yanez Guerra Luis, Egertova Michaela, Elphick Maurice, "The anatomy of neuropeptide gene expression in a pentaradial bilaterian - the starfish Asterias rubens (Phylum Echinodermata)" in 28th Conference of European Comparative Endocrinologists, Leuven, Belgium, 2016

  • Codes CREF : Biologie moléculaire (DI3111), Biologie (DI3100), Sciences exactes et naturelles (DI1000), Zoologie générale (DI3160)
  • Unités de recherche UMONS : Biologie des Organismes Marins et Biomimétisme (S864)
  • Instituts UMONS : Institut des Biosciences (Biosciences)
  • Centres UMONS : Biosys (BIOSYS)

Abstract(s) :

(Anglais) Neuropeptides are ancient neuronal regulators of physiology and behaviour in eumetazoans. Neuropeptide systems are well characterised in vertebrates and some protostomian invertebrates (e.g. Drosophila, C. elegans) but relatively little is known about neuropeptide expression and function in deuterostomian invertebrates, such as echinoderms. As a chordate sister lineage, echinoderms can provide important insights into the evolution of neuropeptide systems. Furthermore, echinoderms provide a unique anatomical perspective on neuropeptide function because they exhibit pentaradial symmetry as adult animals. We are using the starfish Asterias rubens as a model echinoderm system to investigate neuropeptide evolution and function. By analysing neural transcriptome sequence data, we have recently identified forty neuropeptide precursor transcripts in A. rubens. Here we have analysed the anatomical expression patterns in A. rubens of twelve of these neuropeptide precursor transcripts, focusing on precursors of neuropeptides that belong to known bilaterian neuropeptide families: Asterotocin (vasopressin/oxytocin-type), NGFYYamide (NPS/CCAP-type), GnRH, corazonin, TRH, CCK, orexins, luqin, tachykinin, calcitonin and CRH. Using mRNA in situ hybridization methods, expression of all of these precursors was revealed in cells of the circumoral nerve ring and radial nerve cords, with some expressed in both the ectoneural and hyponeural parts (e.g. TRH) and others only in the ectoneural part (e.g. tachykinin). Expression of the GnRH-type precursor appears to be restricted to the nerve ring and cords, but expression of all the other precursors was detected in one or more other regions of the body, including the digestive system and body wall. Expression of numerous precursors was observed in the evertible cardiac stomach, indicating that multiple neuropeptidergic systems are required for control of the unusual extraoral feeding behavior of starfish. Our data provide an anatomical framework for investigating the conservation/divergence of bilaterian neuropeptide function in the context of the highly derived echinoderm bauplan. This work was supported by a BBSRC grant awarded to Maurice R. Elphick.