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2009-09-01 - Article/Dans un journal avec peer-review - Anglais - 7 page(s)

Kong W., Sung D.Y., Shim Y.-H., Bae K.H., Dubois Philippe , Park K., Kim J.H., Seo S.-W., "Efficient intracellular siRNA delivery strategy through rapid and simple two steps mixing involving noncovalent post-PEGylation" in Journal of Controlled Release, 138, 2, 141-147

  • Edition : Elsevier Science, Amsterdam (The Netherlands)
  • Codes CREF : Chimie macromoléculaire (DI1315), Catalyses hétérogène et homogène (DI1334)
  • Unités de recherche UMONS : Matériaux Polymères et Composites (S816)
  • Instituts UMONS : Institut de Recherche en Science et Ingénierie des Matériaux (Matériaux)
Texte intégral :

Abstract(s) :

(Anglais) Two different and well-defined methacrylate-based (co)polymers were employed as a polymeric siRNA delivery system. siRNA, poly(2-(dimethylamino) ethyl methacrylate) homopolymers (PDMAEMA) and poly(2-(dimethylamino) ethyl methacrylate)-b-poly (ethyleneglycol) a-methoxy, ?-methacrylate (PDMAEMA-b-PMAPEG) palm-tree-like copolymer ternary complexes were prepared using a rapid and simple two-step mixing protocol involving noncovalent post-PEGylation, and physicochemical properties including hydrodynamic diameter, zeta-potential and siRNA condensation efficiency were characterized. Transfection efficiency, intracellular uptake, and cytotoxicity of ternary complexes were also evaluated. Ternary complexes provide efficient condensation and compaction of siRNA within the cationic core of complexes. Noncovalent post-PEGylation provides the ternary complexes with enzymatic and serum stability without harming complex formation and condensation of siRNA. Thereby, under an optimal N/P ratio, ternary complexes exhibited brilliant gene silencing efficiency with low cytotoxicity in media containing 10% serum. Confocal microscopy clearly showed efficient and even intracellular uptake of complexes by cells via endocytosis. This study highlights the excellent properties of noncovalent post-PEGylated ternary complexes produced by rapid and simple mixing. Accordingly, these findings suggest that the formation of ternary complexes could be utilized as a safe and effective polymeric siRNA delivery strategy.

Notes :
  • (Anglais) Publié en ligne le 6 mai 2009
  • (Anglais) Lecture en ligne: http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6T3D-4W7B08Y-1-S&_cdi=4944&_user=532054&_pii=S0168365909002624&_origin=search&_coverDate=09%2F01%2F2009&_sk=998619997&view=c&wchp=dGLzVtz-zSkzk&md5=d03591bf1f93cef66f4b8d6c44ff477c
Identifiants :
  • DOI : 10.1016/j.jconrel.2009.04.034