DI-UMONS : Dépôt institutionnel de l’université de Mons

Recherche transversale
(titres de publication, de périodique et noms de colloque inclus)
2016-07-06 - Colloque/Présentation - communication orale - Anglais - 1 page(s)

Neaga Ioan-Ovidiu, Bodoki Ede, Hambye Stéphanie , Blankert Bertrand , Oprean Radu, "Characterization of oligonucleotides-ligand interactions with implications in myotonic dystrophy" in 16th CEEPUS Symposium and Summer School on Bioanalysis , Warsaw, Poland , 2016

  • Codes CREF : Analyse et contrôle pharmaceutique (DI3450), Techniques séparatives (DI2729)
  • Unités de recherche UMONS : Analyse pharmaceutique (M130)
  • Instituts UMONS : Institut des Sciences et Technologies de la Santé (Santé)

Abstract(s) :

(Anglais) Myotonic dystrophy type I, or the Steinert disease, is an autosomal dominant inherited genetic disorder characterized by wasting of the muscles, cataract, cardiac conduction abnormalities and endocrine changes (insulin resistance). At genetic level, the mechanism of the disease is related to a mutation at the DMPK gene characterized by abnormal repeats of the CTG triplet. The transcription of this mutated segment leads to abnormal CUG repeats in the tRNA which in turn can sequestrate an important splicing factor (MBNL-1) thus leading to the symptoms of the disease. Several in vivo studies showed that different small molecules could disrupt this complex by binding competitively to the CUG region and release the MBNL-1 splicing factor restoring its function. An analytical approach could be complementary to in vivo techniques and could facilitate the fast screening of different ligands. The present work illustrates a capillary electrophoresis (CE) method developed for the study of nucleic acids-ligand using pentamidine as the lead compound. Different compounds, mainly antibiotics, were tested and their interaction with the nucleic acids (both DNA and RNA) were estimated in terms of binding constant and stoichiometry. The results suggest that upon additional optimization this CE method can be employed for the rapid screening of potential ligands. In a next step, the most promising ligands may be further tested by in vitro or cells cultures, thus saving time. The research was supported by the PCD, FRMH and CEEPUS CIII-RO-0010-10-1516 scholarships.