DI-UMONS : Dépôt institutionnel de l’université de Mons

Recherche transversale
(titres de publication, de périodique et noms de colloque inclus)
2010-10-21 - Article/Dans un journal avec peer-review - Anglais - 11 page(s)

Michel B., Leroy Baptiste , Stalin Raj V., Lieffrig F., Mast J., Wattiez Ruddy , Vanderplasschen A., Costes B., "Proteomic analyses of koi herpesvirus by mass spectrometry reveal 40 structural proteins" in Journal of General Virology, 91, 2, 452-62

  • Edition : Society for General Microbiology (United Kingdom)
  • Codes CREF : Biochimie (DI3112), Biotechnologie (DI3800)
  • Unités de recherche UMONS : Protéomie et Microbiologie (S828)
Texte intégral :

Abstract(s) :

(Anglais) Koi herpesvirus, also known as cyprinid herpesvirus 3 (CyHV-3), is the aetiological agent of an emerging and mortal disease in common and koi carp. CyHV-3 virions present the characteristic morphology of other members of the order Herpesvirales, being composed of an envelope, a capsid containing the genome and a tegument. This study identified CyHV-3 structural proteins and the corresponding encoding genes using liquid chromatography tandem mass spectrometry-based proteomic approaches. In addition, exponentially modified protein abundance index analyses were used to estimate the relative abundance of the identified proteins in CyHV-3 virions. These analyses resulted in the identification of 40 structural proteins, which were classified based on bioinformatic analyses as capsid (three), envelope (13), tegument (two) and unclassified (22) structural proteins. Finally, a search for host proteins in purified CyHV-3 virions indicated the potential incorporation of up to 18 distinct cellular proteins. The identification of the proteins incorporated into CyHV-3 virions and determination of the viral genes encoding these proteins are key milestones for further fundamental and applied research on this virus.

Identifiants :
  • DOI : 10.1099/vir.0.015198-0