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2016-11-21 - Colloque/Présentation - communication orale - Anglais - 1 page(s)

Contreras Mogollon Angela, Leroy Baptiste , Mariage Pierre-Antoine, Wattiez Ruddy , "Proteomic study of biosynthetic pathways involved in active compounds production in hairy roots" in International PSE symposium Plant Omics ans Biotechnology for Human Health, Gent, Belgium, 2016

  • Codes CREF : Biochimie (DI3112), Phytopharmacie (DI3470), Biotechnologie (DI3800), Biologie moléculaire (DI3111), Biologie (DI3100)
  • Unités de recherche UMONS : Protéomie et Microbiologie (S828)
  • Instituts UMONS : Institut des Biosciences (Biosciences)

Abstract(s) :

(Anglais) Many plants are known to possess a variety of bioactive compounds (secondary metabolites) related to defence against different kind of stress. The large and diverse group of these metabolites includes alkaloids, anthraquinones, anthocyanins, flavanoids, saponins and terpenes, which play an important role in cosmetics, nutritional and pharmaceuticals applications. Hairy roots culture of a number of plants have been established and shown to produce secondary metabolites sometimes more efficiently than natural roots. Hence, researches are still needed to develop appropriate bioreactors to increase biomass productivity but also in order to identify new elicitators improving active compounds production. We are focusing on two particular cases, the ginsenosides production in Panax ginseng and the tanshinones production in Salvia miltiorrhiza. Although many studies performed are related with the importance of these compounds, little is known about biochemical pathways involved in their production and regarding the regulation of those pathways. We tested a variety of biotic and abiotic elicitors. HPLC analysis revealed an increase up to ten fold of the production of the active compounds compared with non-elicitated plants. We optimised proteins extraction strategy as well as LC MS/MS workflow and were able to identified more than 2000 different proteins (at least 2 unique peptides) in a single injection for S. milthiorriza and more than 1500 proteins for P. ginseng, using few mg of fresh material. Differential proteomic analysis are ongoing that will allow better understanding of the molecular mechanisms of biosynthesis the active compounds in these two plants.