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2019-10-01 - Article/Dans un journal avec peer-review - Anglais - 10 page(s)

Devalckeneer Aude , Bouviez Marion , Conotte Raphael , Colet Jean-Marie , "Proton Nuclear Magnetic Resonance (1H NMR) profiling of isolated organs in the snail Helix aspersa maxima" in ecological indicator, 105, 177-187, https://doi.org/10.1016/j.ecolind.2019.05.058

  • Codes CREF : Ecologie (DI3123), Toxicologie [toxines] (DI3236)
Texte intégral :

Abstract(s) :

(Anglais) Abstract Helix aspersa maxima is a terrestrial snail living at the air-ground interface, which makes it an ideal bioindicator candidate to evaluate environmental pollution. In this study, Proton Nuclear Magnetic Resonance (1H NMR) based metabolomics approach was applied on diverse tissue extracts isolated from Helix aspersa individuals. This omics technique allows to characterize in one single spectroscopic measurement the global metabolic changes caused by pollution on the organism and to assess the potential of this species as a pollution indicator. In a first set of experiments conducted on naïve individuals, the main endogenous metabolites were identified. Those included amino and organic acids, Krebs cycle intermediates and a series of osmolytes. Next, a second group of animals was exposed to methomyl, a pesticide used in agriculture, in order to reveal what metabolic pathways were potentially affected by the pollutant. Our results indicate important fluctuations in energetic metabolism and several biomarkers of oxidative stress. In conclusion, this work demonstrates the potential of combining invertebrates models to metabonomics as a powerful tool to predict adverse effects of chemicals.


Mots-clés :
  • (Anglais) InvertebratesSpectroscopyEx vivoToxicity
  • (Anglais) Invertebrates Spectroscopy Ex vivo Toxicity