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2013-01-17 - Article/Dans un journal avec peer-review - Anglais - 10 page(s)

Preira Pascal, Grandne Véronique, Camara Marie, Gabriele Sylvain , Forel Jean-Marie, Theodoly Olivier, "Passive Circulating Cell Sorting by Deformability using a Microfluidic Gradual Filter" in Lab on a Chip - Miniaturisation for Chemistry & Biology, 13, 161-170

  • Edition : Royal Society of Chemistry
  • Codes CREF : Physico-chimie générale (DI1320), Biophysique (DI3113)
  • Unités de recherche UMONS : Laboratoire Interfaces et Fluides complexes (S885)
  • Instituts UMONS : Institut de Recherche sur les Systèmes Complexes (Complexys), Institut des Biosciences (Biosciences)
  • Centres UMONS : Centre d’Innovation et de Recherche en Matériaux Polymères (CIRMAP)
Texte intégral :

Abstract(s) :

(Anglais) The deformability of circulating leukocytes plays an important role in the physiopathology of several diseases like sepsis or acute respiratory distress syndrome (ARDS). We present here a microfluidic method for the passive testing, sorting and separating of non-adherent cell populations by deformability. It consists of microfluidic sieves in series with pore sizes decreasing from the upstream to the downstream. The method capabilities are demonstrated with monocytic cell lines (THP-1) treated by Jasplakinolide (a stabilizer of polymerized actin), LatrunculinA (an inhibitor of actin polymerization), and with the plasma of patients suffering from ARDS. Simple sample injection with standard syringes and pumps makes the method readily adapted for experimentation in hospitals.