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2019-03-21 - Colloque/Présentation - poster - Anglais - page(s)

Delacuvellerie Alice , Cyriaque Valentine , Gobert Sylvie, Benali Samira , Wattiez Ruddy , "FIRST INSIGHTS IN THE ABILITY OF PLASTIC-ASSOCIATED BACTERIAL COMMUNITY TO DEGRADE PETRO-BASED PLASTICS IN MARINE ENVIRONMENT" in Which sustainable future for plastic?, Mons, Belgique, 2019

  • Codes CREF : Microbiologie et protistologie [bacteriol.,virolog.,mycolog.] (DI3130)
  • Unités de recherche UMONS : Protéomie et Microbiologie (S828)
  • Instituts UMONS : Institut des Biosciences (Biosciences)
Texte intégral :

Abstract(s) :

(Anglais) FIRST INSIGHTS IN THE ABILITY OF PLASTIC-ASSOCIATED BACTERIAL COMMUNITY TO DEGRADE PETRO-BASED PLASTICS IN MARINE ENVIRONMENT Alice Delacuvellerie*, Valentine Cyriaque*, Sylvie Gobert**, Samira Benali***, Ruddy Wattiez* * Proteomics and Microbiology Department, University of Mons, 6 Av. du Champ de Mars, 7000 Mons, Belgium (alice.delacuvellerie@umons.ac.be; valentine.cyriaque@umons.ac.be; ruddy.wattiez@umons.ac.be) ** Oceanology Department, University of Liège, 11 Allée du 6 août, 4000 Liège, Belgium (Sylvie.Gobert@ulg.ac.be) *** Polymer and Composite Materials Department, University of Mons,15 Avenue Maistriau, 7000 Mons, Belgium (samira.benali@umons.ac.be) Introduction: Because of the essential role of plastics in our present civilization, the production of plastics increases every year. Most plastics are released in the environment in landfills ending up in the sea, inducing large ecological and health impacts [1]. However, plastics constitute a physical substrate and potential carbon source for microorganisms [2] but little is known about the microbiomes associated to these plastics and their biodegradation. The present study compares the structures of bacterial communities from floating plastics, sediment-associated plastics and sediments from the Mediterranean Sea. Secondly, this study uses environmental samples to investigate the enrichment of potential plastic-degrading bacteria with Low Density PolyEthylene (LDPE), PolyEthylene Terephthalate (PET) and Polystyrene (PS) plastics as the sole carbon source. Methods: The bacterial communities were studied by 16S rRNA sequencing and by DGGE (Denaturing Gradient Gel Electrophoresis). A culture of enrichment with low concentration of conventional carbon (0.05% yeast extract) and plastic (LDPE, PS or PET) as carbon source was performed to select potential bacteria for the plastic degradation. After 2 months, the biofilm microbiome on plastic are studied. Results: The 16S rRNA sequencing results are consistent with previous observations from other sea regions. In addition, we showed that bacterial communities developing on plastics located at different area within the same geographical point (i.e., surface vs sediments) have distinct bacterial communities, with the omnipresence of Bacteroidetes and Gammaproteobacteria. Moreover, the bacterial communities able to form a biofilm on surfaces of synthetic polymers are clearly plastic properties dependent. An enrichment method was used to detect potential specific microbial degraders. Several genera containing hydrocarbon-degrading bacteria species were enriched on several plastics and species association were observed including Alcanivorax borkumensis specifically selected on LDPE films (Fig.1A). Alcanivorax borkumensis and Microbulbifer sp. appear to be enriched in medium with LDPE (Fig.1B), and the LDPE surface shows signs of degradation (Fig.1C). Interestingly, other hydrocarbon-degrading bacteria, such as Marinobacter and Arenibacter, are also enriched with LDPE and PET as carbon sources, implying that these bacteria are potential players/candidates in plastic degradation.