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2020-03-14 - Article/Dans un journal avec peer-review - Anglais - page(s)

Fanfone Deborah, Stanicki Dimitri , Nonclercq Denis , Port Marc, Vander Elst Luce , Laurent Sophie , Muller Robert , Saussez Sven , Burtea Carmen , "Molecular Imaging of Galectin-1 Expression as a Biomarker of Papillary Thyroid Cancer by Using Peptide-Functionalized Imaging Probes" in Biology, 9, 53, 1-25

  • Edition : Multidisciplinary Digital Publishing Institute (MDPI) (Switzerland)
  • Codes CREF : Histologie (DI3212), Résonance magnétique nucléaire (biophysique) (DI131B), Sciences biomédicales (DI3200), Endocrinologie (DI3322), Biochimie pharmaceutique (DI3491), Chimie organique (DI1313), Imagerie médicale, radiologie, tomographie (DI3243), Cancérologie (DI3349), Biologie cellulaire (DI311D)
  • Unités de recherche UMONS : Chimie générale, organique et biomédicale (M108), Anatomie et Biologie cellulaire (M112), Histologie (M118)
  • Instituts UMONS : Institut des Sciences et Technologies de la Santé (Santé), Institut des Biosciences (Biosciences)
  • Centres UMONS : Centre de Recherche en Microscopie et Imagerie Médicale (CMMI)
Texte intégral :

Abstract(s) :

(Anglais) Thyroid cancers are the most frequent endocrine cancers and their incidence is increasing worldwide. Thyroid nodules occur in over 19%–68% of the population, but only 7%–15% of them are diagnosed as malignant. Diagnosis relies on a fine needle aspiration biopsy, which is often inconclusive and about 90% of thyroidectomies are performed for benign lesions. Galectin-1 has been proposed as a confident biomarker for the discrimination of malignant from benign nodules. We previously identified by phage display two peptides (P1 and P7) targeting galectin-1, with the goal of developing imaging probes for non-invasive diagnosis of thyroid cancer. The peptides were coupled to ultra-small superparamagnetic particles of iron oxide (USPIO) or to a near-infrared dye (CF770) for non-invasive detection of galectin-1 expression in a mouse model of papillary thyroid cancer (PTC, as the most frequent one) by magnetic resonance imaging and fluorescence lifetime imaging. The imaging probes functionalized with the two peptides presented comparable image enhancement characteristics. However, those coupled to P7 were more favorable, and showed decreased retention by the liver and spleen (known for their galectin-1 expression) and high sensitivity (75%) and specificity (100%) of PTC detection, which confirm the aptitude of this peptide to discriminate human malignant from benign nodules (80% sensitivity, 100% specificity) previously observed by immunohistochemistry.

Identifiants :
  • DOI : 10.3390/biology9030053

Mots-clés :
  • (Anglais) CF770
  • (Anglais) functionalized imaging probes
  • (Anglais) peptides
  • (Anglais) fluorescence lifetime imaging
  • (Anglais) thyroid cancer
  • (Anglais) galectin-1
  • (Anglais) ultra-small superparamagnetic particles of iron oxide
  • (Anglais) magnetic resonance imaging