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2013-01-27 - Colloque/Présentation - poster - Anglais - 1 page(s)

Rubio Magnieto Jenifer , Thomas amandine, Clement Sebastien, Lazzaroni Roberto , Surin Mathieu , "Insights into the self-assembly of cationic conjugated polythiophenes – single-stranded DNA hybrid structures" in European-Winter School on Physical Organic Chemistry (E-WiSPOC), Bressanone, Italy, 203

  • Codes CREF : Chimie structurale (DI1317), Chimie des polymères de synthèse (DI131C)
  • Unités de recherche UMONS : Chimie des matériaux nouveaux (S817)
  • Instituts UMONS : Institut de Recherche en Science et Ingénierie des Matériaux (Matériaux)
  • Centres UMONS : Centre d’Innovation et de Recherche en Matériaux Polymères (CIRMAP)

Abstract(s) :

(Anglais) Insights into the self-assembly of cationic conjugated polythiophenes – single-stranded DNA hybrid structures J. Rubio-Magnieto*a, A. Thomasb, S. Clémentb, R. Lazzaronia, M. Surin*a aLaboratory for Chemistry of Novel Materials, Center for Innovation in Materials and Polymers, University of Mons - UMONS, 20 Place du Parc, B-7000 Mons, Belgium. Tel: +32 (0)65 37 38 65. bInstitut Charles Gerhardt - UMR5253, Equipe Chimie Moléculaire et Organisation du Solide, Université de Montpellier 2 - CC1701, Place Eugène Bataillon, F-34095 Montpellier Cedex 05, France. e-mail: jenifer.rubiomagnieto@umons.ac.be The binding between different biomolecules as DNA and synthetic water-soluble cationic π-conjugated polymers (CPs) has attracted many researchers in the last decade.1 In this frame, the combination of the recognition properties of the DNA and the interesting optical properties of the CPs are crucial. Taking into account this advantage, we designed a series of cationic polythiophenes (PTs) with different ionic groups able to bind with the negatively charged backbone of oligonucleotides. Here, we present our recent studies on the self-assembly of four achiral PTs with various specific DNA, by means of circular dichroism and UV-Vis spectroscopy. The effect of the DNA length, molar concentration ratio, temperature and DNA sequence was studied. We observe that, in some specific cases, the DNA changes its right-handed initial conformation upon binding with PT. Very remarkably, an induced CD signal in the PT absorption region appears, suggesting that the polymers adopt a chiral conformation within the hybrid supramolecular complex. Thus, these early results indicate the possibility of using these cationic polymers and oligonucleotides in order to establish the structure-properties relationships and the self-assembly mechanisms to evolve towards optical (bio)sensors. Figure 1. a) Structures of the cationic water-soluble PTs used in this study. b) CD spectra of a typical B-form DNA (black line) and ssDNAd(X)n : P3HT-PMe3 1 : 1 (red, green and blue lines). Measurements were done in TE buffer, pH 7.4 at 20 ºC. [ssDNAd(X)n] = 6.67 µM in all spectra. References 1. C. Zhu, L. Liu, Q. Yang, F. Lv, S. Wang, Chem. Rev. 2012, 112, 4687-4735; B. S. Gaylord, A. J. Heeger and G. C. Bazan, Proc. Natl. Acad. Sci., 2002, 99, 10954-10957. 2. H. A. Ho, A. Najari and M. Leclerc, Acc. Chem. Res., 2008, 41, 168-178; S. Clément, A. Tizit, S. Desbief, A. Mehdi, J. De Winter, P. Gerbaux, R. Lazzaroni and B. Boury, J. Mater. Chem., 2011, 21, 2733-2739. Acknowledgements: This work was funded by the Fonds National de la Recherche Scientifique (F.R.S.-FNRS) via the FRFC - BINDER project.