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2009-01-01 - Article/Dans un journal avec peer-review - Anglais - 18 page(s)

Burtea Carmen , Laurent Sophie , Port M., Lancelot E., Ballet S., Rousseaux O., Toubeau Gérard, Vander Elst Luce , Corot C., Muller Robert , "Magnetic Resonance Molecular Imaging of Vascular Cell Adhesion Molecule-1 Expression in Inflammatory Lesions Using a Peptide-Vectorized Paramagnetic Imaging Probe" in Journal of Medicinal Chemistry, 52, 15, 4725-4742

  • Edition : American Chemical Society, Washington (DC)
  • Codes CREF : Histologie (DI3212), Résonance magnétique nucléaire (biophysique) (DI131B), Sciences biomédicales (DI3200), Cardiologie et circulation (DI3321), Biologie moléculaire (DI3111), Chimie organique (DI1313), Imagerie médicale, radiologie, tomographie (DI3243)
  • Unités de recherche UMONS : Chimie générale, organique et biomédicale (M108), Histologie (M118)
  • Instituts UMONS : Institut des Sciences et Technologies de la Santé (Santé), Institut des Biosciences (Biosciences)
  • Centres UMONS : Centre de Recherche en Microscopie et Imagerie Médicale (CMMI)
Texte intégral :

Abstract(s) :

(Anglais) The vascular cell adhesion molecule-1 (VCAM-1) has distinct roles in inflammatory cell recruitment to the damaged vessel wall. In the present work, a cyclic heptapeptide phage displayed library was screened in vitro during four rounds of biopanning. On the basis of K-d and IC50 values, a peptide (encoded as R832) was selected for in vitro and in vivo validation. After conjugation to Gd-DOTA, VCAM-I imaging was assessed by MRI on a model of T cell mediated hepatitis, induced in mice by concanavalin A. On histological samples, the location of biotinylated R832 (R832-Bt) around liver veins in hepatitis resembles the pattern of M RI enhancement. Gd-DOTA-R832 was then assessed oil ApoE(-/-) mice and produced ail important signal enhancement of the aortic wall, while R832-Bt interacted with morphologic structures comparable to those marked by anti-VCAM-1 antibody. In conclusion, the in vitro and in vivo evaluation of peptide R832 suggests a specific interaction with the targeted biomolecule. Its conjugation to imaging reporters could assist the diagnosis of inflammatory diseases.

Identifiants :
  • DOI : 10.1093/cvr/cvm115
  • ISSN : 0022-2623

Mots-clés :
  • (Anglais) atherosclerosis
  • (Anglais) peptides
  • (Anglais) MRI contrast agents
  • (Anglais) molecular imaging
  • (Anglais) vulnerable plaque