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2009-03-30 - Article/Dans un journal avec peer-review - Anglais - 7 page(s)

Leroy Baptiste , Viart S, Trinchero N, Roupie V., Govaerts M., Letesson J.J., Huygen K., Wattiez Ruddy , "Use of Mycobacterium avium subsp. paratuberculosis specific coding sequences for serodiagnosis of bovine paratuberculosis" in Veterinary Microbiology, 135, 3-4, 313-319

  • Edition : Elsevier Science, Amsterdam (The Netherlands)
  • Codes CREF : Biochimie (DI3112), Biotechnologie (DI3800), Microbiologie et protistologie [bacteriol.,virolog.,mycolog.] (DI3130)
  • Unités de recherche UMONS : Protéomique et Microbiologie (S828)
Texte intégral :

Abstract(s) :

(Anglais) In this study, the finished complete genome of Mycobacterium avium subsp. paratuberculosis (Map) was screened for specific coding sequences that could be very valuable in the design of a sensitive and specific Map detection serological assay. Eighty-seven Map-specific sequences were retained. Among these, three candidate antigens have been analysed for their serodiagnostic potential. These antigens were selected on the basis of their putative immunogenicity as predicted by in silico analysis. The antigens were cloned in Escherichia coli, expressed, and purified before testing in an antibody detection ELISA test, using a well characterized panel of 18 and 48 sera from Map infected and uninfected cattle, respectively. Two of these antigens, antigen 6 and MAP1637c, yielded in our conditions a sensitivity of 72% and 82%, respectively, for a specificity of 98%. It is particularly noticeable that, when probed with the same serum panel, the most widely used European paratuberculosis commercial seroassay (Pourquier test) yielded a sensitivity of 72% for a specificity of only 92%.

Notes :
  • (Anglais) Publié en ligne le 24 septembre 2008
  • (Anglais) Lecture en ligne: http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6TD6-4THJGKF-1-7&_cdi=5190&_user=532054&_pii=S0378113508004422&_origin=search&_coverDate=03%2F30%2F2009&_sk=998649996&view=c&wchp=dGLbVlz-zSkzV&md5=2d837274b4bdb056606d6268bfe836bf
Identifiants :
  • DOI : 10.1016/j.vetmic.2008.09.065