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Recherche transversale
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2018-07-12 - Colloque/Présentation - poster - Anglais - 1 page(s)

Wells Mathilde , Hambye Stéphanie , Blankert Bertrand , "Isolation and identification strategies of potential antimalarial components from toad venom (bufo marinus) using thin-layer chromatography." in 2nd International Symposium on Advances in Pharmaceutical Analysis, Lille, France, 2018

  • Codes CREF : Analyse et contrôle pharmaceutique (DI3450), Sciences pharmaceutiques (DI3400), Techniques séparatives (DI2729)
  • Unités de recherche UMONS : Analyse pharmaceutique (M130)
  • Instituts UMONS : Institut des Sciences et Technologies de la Santé (Santé)
Texte intégral :

Abstract(s) :

(Anglais) With more than 216 million cases and 445.000 deaths reported by the World Health Organization (WHO) in 2016, malaria remains a major concern for public health organizations. 91 countries are affected with more than 800 million people at risk of infection. Unfortunately, resistances to most antimalarial drugs emerge and create a real worry, leading to an urgent need for new therapeutic candidates [1]. Animals’ venoms and secretions constitute a wide reservoir of molecules with many potential therapeutic activities [2]. In this frame, toad venoms have sparked more and more interest in scientists. The main purpose of this project aims to isolate compounds from the toad venom and to test whether they possess a potential antiplasmodial activity. However, the first step, will consist to extract and collect compounds from crude venoms. To pursue this goal, thin-layer chromatography (TLC) is able to provide a possible isolation step. Firstly, various extraction solvents will be tested, to obtain raw venom extracts. Secondly, we proceed to the separation of the different components contained in the raw extracts by TLC coupled to mass spectrometry. Two different types of stationary phases are studied: ungrafted silica plates and C18-grafted silica plates, under multiple mobile phases conditions. Chromatographic resolution of each combination is assessed. Although TLC offers a range of advantages such as rapid analysis time and low solvent consumption, lack of reproducibility remains a concern. To solve this problem, we evaluated the sample deposit, the migration and final drying steps as improvement parameters [3]. References 1) Malaria Report OMS, 2017. 2) Rodriguez, C., et al., Toxins and pharmacologically active compounds from species of the family Bufonidae (Amphibia, Anura), Journal of Ethnopharmacology, 2017 (198), 235 – 254. 3) Ciura, K., et al., Thin layer chromatography in drug discovery process, J. of chromatography A, 2017 (1520), 9 – 22.

Mots-clés :
  • (Anglais) thin-layer chromatography
  • (Anglais) Toad venom
  • (Anglais) Antiplasmodial