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2013-05-16 - Colloque/Présentation - poster - Anglais - 1 page(s)

Pardo Antonelle , Mespouille Laetitia , Dubois Philippe , Blankert Bertrand , Duez Pierre , "MOLECULARLY IMPRINTED POLYMERS: TOWARDS RECEPTOR MIMICS FOR DRUG DISCOVERY" in Belgian Polymer Group , Houffalize, Belgium, 2013

  • Codes CREF : Chimie des polymères de synthèse (DI131C), Chimie analytique (DI1314), Pharmacognosie (DI3410), Sciences pharmaceutiques (DI3400), Techniques séparatives (DI2729)
  • Unités de recherche UMONS : Analyse pharmaceutique (M130), Chimie thérapeutique et Pharmacognosie (M136), Matériaux Polymères et Composites (S816)
  • Instituts UMONS : Institut de Recherche en Science et Ingénierie des Matériaux (Matériaux), Institut des Sciences et Technologies de la Santé (Santé)
Texte intégral :

Abstract(s) :

(Anglais) Molecularly imprinted polymers (MIPs) are molecular recognition materials composed of specific cavities designed upon a template molecule. They are produced by copolymerization of functional monomers and a high fraction of cross-linking agents in the presence of a template molecule. This work aims at developing a selective chromatographic media based on MIPs for the screening of active components from natural products. In the field of drug discovery, rigid imprint formation is not required since the MIPs should be able to recognize structural analogues of the template. In order to find a compromise between flexibility and rigidity, quercetin imprinted polymers (Qu MIPs) (Fig. 1) were prepared by modifying the synthesis reagents composition. Corresponding control polymers (non-imprinted polymers, NIPs), formulated in a similar fashion, without introduction of the quercetin template molecule, were produced, treated and studied similarly than MIPs. Bulk polymerization (BP), precipitation polymerization (PP) and suspension polymerization (SP) were investigated to obtain optimal chromatographic materials. Particles size and shape were characterized by scanning electron microscopy (SEM) and/or transmission electron microscopy. The MIPs were finally evaluated as sorbents for solid-phase extraction (SPE) and HPLC. The HPLC selectivity study towards analogues and non-analogues of the template molecule quercetin (Qu) allowed to confirm the modulation of flexibility thanks to original synthesis strategies. Successful imprinting was confirmed by SPE since the retention of quercetin appeared distinctly superior on MIPs than on NIPs. The best SPE results were obtained for MIPs prepared by the SP method. The micrographs obtained by SEM revealed a large distribution of sizes and irregular shape of the particles prepared by BP. Oppositely, spherical beads having a homogeneous size distribution were obtained by SP. The SP approach appeared therefore as the most attractive because it produces polymer beads with a considerable yield and improved chromatographic characteristics.

Mots-clés :
  • (Anglais) Molecularly Imprinted Polymers
  • (Anglais) Screening
  • (Anglais) Pharmaceutical analysis
  • (Anglais) Solid-phase extraction
  • (Anglais) Molecular recognition