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2019-04-15 - Article/Dans un journal avec peer-review - Anglais - 9 page(s)

Loyez Médéric , Larrieu Jean-Charles, Chevineau Samia , Remmelink Myriam, LEDUC Dimitri, Bondue Benjamin, Lambert Pierre, Devière Jacques, Wattiez Ruddy , Caucheteur Christophe , "In situ cancer diagnosis through online plasmonics" in Biosensors and Bioelectronics, 131, 104-112

  • Edition : Elsevier (United Kingdom)
  • Codes CREF : Biochimie (DI3112)
  • Unités de recherche UMONS : Protéomie et Microbiologie (S828), Electromagnétisme et Télécommunications (F108)
  • Instituts UMONS : Institut des Biosciences (Biosciences)
Texte intégral :

Abstract(s) :

(Anglais) Most cancer diagnoses rely on biomarkers detection. This could be improved if directly conducted in suspicious cancer spots, preventing the need for biopsy. Lung cancer remains a perfect study-case for such a development, as it is generally detected at advanced stage and is in the need for early diagnosis techniques. To this aim, we have designed a minimally invasive catheter-embedded biosensor. It combines a specific grating structure photo- imprinted in a telecommunication-grade optical fiber and an overlay made of a thin metal coating on which receptors are grafted, yielding plasmonic coupling. Our optrode targets a type of cytokeratins, overexpressed at the surface of cancer cells. It was assayed ex vivo in resected lung tissues collected from a dozen of patients. Biosensing responses were confirmed by immunohistochemistry, conducted on the same samples. In addition to accurate biosensing, our gratings inherently enable force-sensing features, which also allow a fine positioning of the probe in the tissue. Finally, the in vivo navigation of the bronchoscope-embedded sensor was validated into pig lungs. These achievements are a critical milestone towards the development of this micro/nano biosensor as a cost-effective and weakly invasive diagnostic tool for applications in areas of critical access such as brain, liver or prostate.

Mots-clés :
  • (Anglais) Biomarker
  • (Anglais) SPR
  • (Anglais) Plasmonics
  • (Anglais) TFBG
  • (Anglais) Optical Fiber
  • (Anglais) Biosensing