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2014-07-18 - Colloque/Présentation - poster - Anglais - 1 page(s)

Nachtergael Amandine , Poivre Mélanie, Belayew Alexandra , Duez Pierre , "A preliminary assessment of traditional Chinese herbs cytotoxicity and genotoxicity" in The 3rd Annual Meeting of the GP-TCM Research Association and the 5th Annual Conference of the Specialty Committee of TCM Pharmaceutical Analysis of WFCMS, Nanjing, Chine, 2014

  • Codes CREF : Chimie analytique (DI1314), Pharmacognosie (DI3410), Sciences pharmaceutiques (DI3400), Toxicologie pharmaceutique (DI3440)
  • Unités de recherche UMONS : Chimie thérapeutique et Pharmacognosie (M136)
  • Instituts UMONS : Institut des Sciences et Technologies de la Santé (Santé)

Abstract(s) :

(Anglais) Herbal medicines are widely used around the world, either for primary health care or as complementary medicines. These herbal medicines are generally considered as safe and reliable. However, herbs can sometimes cause serious health troubles. For example, the aristolochic acid nephropathy (AAN), a progressive renal interstitial fibrosis frequently associated with urothelial malignancies, was reported in a Belgian cohort after the intake of slimming pills inadvertently containing an Aristolochia species, rich in aristolochic acids (AA), known to be genotoxic through the generation of aristolactams which yield highly persistent and non-or poorly-repaired DNA adducts. Interestingly, the Belgian cases are remarkable by the rapid onset of the pathology. Available records indicate that the supposedly slimming pills prescription included roots of Stephania tetrandra, that were in fact adulterated by the roots of an Aristolochia species (probably A. fangchi), and also bark of Magnolia officinalis. The goal of the present study is to evaluate on a human intestinal epithelial cell line the cytotoxicity and genotoxicity of Aristolochia baetica and Magnolia officinalis aqueous and methanolic extracts, alone or in combination, by a MTT cell viability assay and by the detection and quantification of the phosphorylated histone gamma-H2AX (whole cell ELISA and Immunocytochemistry). The genotoxicity of the association of Aristolochia baetica and Magnolia officinalis extracts is significantly higher compared to the individual plant extracts. The high potentiation of AAs genotoxicity by Magnolia officinalis could then be an explanatory factor for the “Chinese herbs nephropathy” cases, observed in Belgium in the 1990s. Although this would be a very interesting example of indirect genotoxicity, this observation warrants further studies to establish the precise role of the Magnolia officinalis extracts and to identify the compounds responsible for the observed co-genotoxicity.