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2016-02-25 - Colloque/Présentation - poster - Anglais - 1 page(s)

Chevineau Samia , Ribaut Clotilde, Wattiez Ruddy , "Specific surface biomarker determination by LC-MS/MS for lung cancer diagnosis using optical fiber biosensor" in 11th Annual Biomarkers Congress 2016, Manchester, United Kingdom, 2016

  • Codes CREF : Biochimie (DI3112)
  • Unités de recherche UMONS : Protéomie et Microbiologie (S828)
  • Instituts UMONS : Institut des Biosciences (Biosciences)
Texte intégral :

Abstract(s) :

(Anglais) In cancer care, optical fiber biosensors have the potential to yield weakly invasive in vivo protein detection that would considerably enhance the rapidity of the diagnosis while avoiding severe operations. This would be of considerable interest in areas of critical access such as the distal bronchial tubes where a chirurgical intervention to obtain diagnosis might be avoided, causing less disturbances for the patients. To achieve a quick diagnosis using an optical fiber biosensor, lung cancer biomarkers specific to the surface of the tumors need to be determined. Fresh biopsies of lung tumors and their non-tumorous lung tissue control were collected from the Laboratory of Anatomic Pathology at the Erasmus Hospital in Brussels. All surface proteins were labelled without discrimination using a commercial Biotin tag (EZ-Link™ Sulfo-NHS-SS-Biotin). These surface proteins were recovered using a High Capacity NeutrAvidin™ Agarose Resin and subsequently analyzed using a LC-MS/MS approach with an AB SCIEX TripleTOF™ 5600. After analysis of the data, two potential specific candidate biomarkers have been determined: Cytokeratin-17 and Protein disulfide-isomerase A6, and are currently ongoing further validation by Western-Blot and Immunohistochemistry. This work is supported by the ARC named “PRotein dEtection for in vivo DIagnosis using CaTheterIc Optical fibre bioseNsors (PREDICTION)” (n° AUWB-2012-12/17-UMONS/ULB-N°1).