DI-UMONS : Dépôt institutionnel de l’université de Mons

Recherche transversale
(titres de publication, de périodique et noms de colloque inclus)
2018-07-03 - Colloque/Présentation - poster - Anglais - 1 page(s)

Carette Julie , Nachtergael Amandine , Rasamiravaka Tsiry, El Jaziri Mondher, Duez Pierre , "Prospects on natural molecules disturbing Pseudomonas aeruginosa biofilms architecture" in The 6th Annual Meeting of the Good Practice in Traditional Chinese Medicine Research Association, London, United Kingdom, 2018

  • Codes CREF : Pharmacognosie (DI3410)
  • Unités de recherche UMONS : Chimie thérapeutique et Pharmacognosie (M136)
  • Instituts UMONS : Institut des Sciences et Technologies de la Santé (Santé)

Abstract(s) :

(Anglais) Prospects on natural molecules disturbing the biofilm architecture from Pseudomonas aeruginosa Julie Carette, Amandine Nachtergael, Mondher El Jaziri and Pierre Duez Department of Therapeutical Chemistry and Pharmacognosy, Université de Mons, Avenue Maistriau 19, Bâtiment 6, 7000 Mons, Belgium Laboratory of Plant Biotechnology, Université Libre de Bruxelles, rue des Professeurs Jeener et Brachet 12, 6041 Gosselies, Belgium Nowadays, a serious threat in healthcare is the antibiotic resistances. Across the world, antibiotic resistances reach an unsafe level. If the situation doesn’t change, benign infections will reveal itself dangerous. The increase and spread of the antibiotics resistance is a known problem that it must be urgently solved. WHO provide a priority list divided in three group for bacteria antibiotic resistances according to the urgency of needed for new treatement. Pseudomonas aeruginosa is part of the group 1 :level « critical ». This is a gram negative and opportunistic pathogenic bacteria knowed to be a major cause of various infections because of its ability to form biofilms in several environments. The aims of this work is to answer to the necessity of discover and develop natural antimicrobial agents. The strategy we choose is to disturb the architecture of the biofilm to improve the penetration of antimicrobial agents. Thanks to the CDC bioreacteur, we form a biofilm with in vivo conditions and electronics microscopy permit to caracterized the biofilm architecture. We also use a microfluidics cells to study the effect on the architecture of biofilm formed of several natural moleccules known and news, we additionaly employ Nunc TSP plate to perform assay of natural molecules on biofilm in formation. With this results, we try to study a new, easier and faster method to measure the disruppting of architecture of the biofilm with the help of electrical potential. We also aim to discover the mechanism and interaction targets of the natural molecules on the biofilm.