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2009-01-01 - Article/Dans un journal avec peer-review - Anglais - 10 page(s)

Kovbasyuk L., Moucheron Cécile, Dubois Philippe , Kirsch-De-Mesmaeker A., "Probing the interaction between DNA and cells transfection polymers with luminescent Ru(II) complexes" in New Journal of Chemistry, 33, 5, 1047-1056

  • Edition : Royal Society of Chemistry, Cambridge (United Kingdom)
  • Codes CREF : Chimie macromoléculaire (DI1315), Catalyses hétérogène et homogène (DI1334)
  • Unités de recherche UMONS : Matériaux Polymères et Composites (S816)
  • Instituts UMONS : Institut de Recherche en Science et Ingénierie des Matériaux (Matériaux)
Texte intégral :

Abstract(s) :

(Anglais) The luminescence properties of RuII complexes [Ru(TAP)2(phen)]Cl2 (1), [Ru(TAP)2(TPAC)]Cl2 (2) and [Ru(phen)2(TPAC)]Cl2 (3) (TAP = 1,4,5,8-tetraazaphenanthrene and TPAC = tetrapyrido-acridine) were used for probing the interactions of cell transfection (co)polymers, such as poly[2-dimethylaminoethyl-methacrylate] (PDMAEMA) and poly[2-dimethylaminoethylmethacrylate]-b-poly[(ethylene glycol-a-methylether-?-methacrylate] (P(DMAEMA-b-MAPEG)), with different polynucleotides (poly[(dA-dT)]2 and poly[(dG-dC)]2) and nucleic acids (calf thymus DNA and pBR322 plasmid DNA). It turned out that 1 was not a useful probe because its affinity constant for DNA was too weak. Moreover, 3 was also excluded because DNA-Ru complex aggregates complicated the interpretation of the data. 2 was the only candidate that could be used as a luminescent probe of "DNA?polymer" self-assemblies that would penetrate cell membranes. Different (co)polymer/polynucleotide ratios were tested with complex 2 by luminescence measurements. The results showed that 2 is an interesting probe, which is very sensitive to changes in the polynucleotide double helix structure that are induced by interactions with the synthetic (co)polymer. Moreover, complex 2 has a mode of action of emission that is different from that of the classically used ethidium bromide, i.e. an increase in emission when certain "polymer/DNA" ratios are reached during the titration of DNA with the polymer.

Identifiants :
  • DOI : 10.1039/b815193h