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2017-06-16 - Colloque/Présentation - poster - Anglais - 1 page(s)

Trevisan Marie , Fossepre Mathieu , Ulrich S., Surin Mathieu , "Chiroptical properties of supramolecular assemblies containing adenosine phosphates" in 16th International Conference on Chiroptical Spectroscopy 2017, Rennes, France, 2017

  • Codes CREF : Chimie macromoléculaire (DI1315), Spectroscopie [électromagnétisme, optique, acoustique] (DI1255), Chimie analytique (DI1314)
  • Unités de recherche UMONS : Chimie des matériaux nouveaux (S817)
  • Instituts UMONS : Institut de Recherche en Science et Ingénierie des Matériaux (Matériaux), Institut des Biosciences (Biosciences)
Texte intégral :

Abstract(s) :

(Anglais) Enzyme-Responsive Materials (ERMs) have become an emerging research topic with potential applications in drug delivery, sensing and regenerative medicine. Most ERMs studied so far were dealing with peptide-based materials, whereas those involving nucleic acids have been scarcely explored. In this context, we aim at studying the enzymatic activity towards supramolecular hybrids containing a biomolecular substrate (nucleotide, DNA) by circular dichroism (CD) spectroscopy. For this, we are taking advantage of the induced circular dichroism (ICD) signals observed for supramolecular assemblies in which (oligo)nucleotides are interacting with a π-conjugated achiral molecule. These induced CD signals of supramolecular assemblies in the absorbance range of the achiral molecule are extremely sensitive to the changes in the nucleic acids microenvironment, such as effects of DNA sequence and length. One of the systems selected for this study is an assembly of adenosine phosphate (AP) with Guanidinium-BisPyrene (GBP), to follow the ATP hydrolysis by an alkaline phosphatase. Preliminary experiments show that the ICD signals of solutions composed of assemblies of GBP with AMP, or ADP, or ATP strongly differ at a given molar ratio. Moreover, we observed that a mixture of 2 or 3 forms of AP gave CD spectra distinctive enough to be correlated to the proportion of the different AP present in solution. Those results suggest that the hydrolysis by alkaline phosphatase could be monitored by circular dichroism spectroscopy.