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2007-06-01 - Article/Dans un journal avec peer-review - Anglais - page(s)

Kowaljow Valeria, Marcowycz Aline, Ansseau Eugénie , Conde Cecilia B., Sauvage Sébastien, Mattéotti Christel, Arias Cristina, Corona E. Daniel, Nuñez Nicolas G., Leo Oberdan, Wattiez Ruddy , Figlewicz Denise, Laoudj-Chenivesse Dalila, Belayew Alexandra , Coppée Frédérique , Rosa Alberto L., "The DUX4 gene at the FSHD1A locus encodes a pro-apoptotic protein" in Neuromuscular Disorders, 17, 8, 611-23

  • Edition : Elsevier Science
  • Codes CREF : Biochimie (DI3112), Biologie moléculaire (DI3111), Acides nucléiques, synthèse des protéines (DI311A), Pathologies particulières (DI3370), Génie génétique (DI311H)
  • Unités de recherche UMONS : Biologie moléculaire (M122), Protéomique et Microbiologie (S828)
Texte intégral :

Abstract(s) :

(Anglais) Facioscapulohumeral muscular dystrophy (FSHD) patients carry contractions of the D4Z4-tandem repeat array on chromosome 4q35. Decrease in D4Z4 copy number is thought to alter a chromatin structure and activate expression of neighboring genes. D4Z4 contains a putative double-homeobox gene called DUX4. We identified DUX4 mRNAs in cells transfected with genomic fragments containing the DUX4 gene. Using RT-PCR we also recognized expressed DUX4 mRNAs in primary FSHD myoblasts. Polyclonal antibodies raised against specific DUX4 peptides detected the DUX4 protein in cells transfected with D4Z4 elements. DUX4 localizes in the nucleus of cells transfected with CMV–DUX4 expression vectors. A DUX4-related protein is endogenously expressed in nuclei of adult and fetal human rhabdomyosarcoma cell lines. Overexpression of DUX4 induces cell death, induces caspase 3/7 activity and alters emerin distribution at the nuclear envelope. We propose that DUX4-mediated cell death contributes to the pathogenic pathway in FSHD.

Notes :
  • (Anglais) Lecture en ligne: http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6T9T-4P2J330-1-1&_cdi=5123&_user=532054&_pii=S0960896607001216&_origin=search&_coverDate=08%2F31%2F2007&_sk=999829991&view=c&wchp=dGLzVzz-zSkzS&md5=bdd85671547bb6fc73a4c0e4cef105bb
  • (Anglais) Publié le 27 juin 2007
Identifiants :
  • DOI : 10.1016/j.nmd.2007.04.002