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2018-01-01 - Article/Dans un journal sans peer-review - Anglais - 30 page(s)

Léonet Aline, Delroisse Jérôme , Schuddinck Christopher, Wattiez Ruddy , Jangoux Michel, Eeckhaut Igor , "Thioredoxins induce oocyte maturation in holothuroids (Echinodermata)" in BioRxiv: The preprint server for biology

  • Codes CREF : Biologie moléculaire (DI3111), Biologie (DI3100), Sciences exactes et naturelles (DI1000), Zoologie générale (DI3160)
  • Unités de recherche UMONS : Biologie des Organismes Marins et Biomimétisme (S864)
  • Instituts UMONS : Institut des Biosciences (Biosciences)
  • Centres UMONS : Biosys (BIOSYS)
Texte intégral :

Abstract(s) :

(Anglais) Chromatographic fractions of a rough extract of echinoid spawn (REES) has been demonstrated to efficiently induce oocyte maturation in aspidochirote holothuroids. The method is so efficient that it is now used in holothuriculture to get fertilised eggs even outside the reproductive period of the aquacultured species. We here isolate and identify from echinoid spawns the molecule responsible for the induction of the oocyte maturation in the Mediterranean holothuroid Holothuria tubulosa and the Indo-Pacific Holothuria scabra. The use of proteinase K and dialysing membranes indicates that the active molecule is a protein with a molecular weight superior to 12,000 kDa. The active molecule has been isolated on G- 100 Sephadex chromatography column. Active chromatographic fractions include seven proteins identified with nanoLC-MS/MS technique. One of them, identify as a thioredoxin-2 and to which the name Trx-REES has been given, leads up to levels of maturation similar to those obtained with the REES and with a commercial thioredoxin extracted from Escherichia coli. Occurrence of thioredoxin in REES was confirmed by immunoblot analysis, and the maturation-inducing properties of thioredoxin were positively checked in using anti- thioredoxin antibodies. A peptide of 6 AAs corresponding to the active site of Trx-REES, composed of WCNPCK, was synthesised and its efficiency in holothuroid oocyte maturation tested. At some concentrations, the peptide was 1.2 times more active than the REES.