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2017-10-06 - Article/Dans un journal avec peer-review - Anglais - 17 page(s)

Fanfone Deborah , Despretz Nadège, Stanicki Dimitri , Rubio Magnieto Jenifer , Fossepre Mathieu , Surin Mathieu , Rorive Sandrine, Salmon Isabelle, Vander Elst Luce , Laurent Sophie , Muller Robert , Saussez Sven , Burtea Carmen , "Toward a new and noninvasive diagnostic method of papillary thyroid cancer by using peptide vectorized contrast agents targeted to galectin-1" in Medical Oncology

  • Edition : Humana Press (United States)
  • Codes CREF : Histologie (DI3212), Sciences biomédicales (DI3200), Biologie moléculaire (DI3111), Imagerie médicale, radiologie, tomographie (DI3243), Cancérologie (DI3349), Biologie cellulaire (DI311D)
  • Unités de recherche UMONS : Chimie des matériaux nouveaux (S817), Chimie générale, organique et biomédicale (M108), Anatomie et Biologie cellulaire (M112)
  • Instituts UMONS : Institut des Sciences et Technologies de la Santé (Santé), Institut des Biosciences (Biosciences)
  • Centres UMONS : Centre de Recherche en Microscopie et Imagerie Médicale (CMMI)
Texte intégral :

Abstract(s) :

(Anglais) The incidence of papillary thyroid cancer has increased these last decades due to a better detection. High prevalence of nodules combined with the low incidence of thyroid cancers constitute an important diagnostic challenge. We propose to develop an alternative diagnostic method to reduce the number of useless and painful thyroidectomies using a vectorized contrast agent for Magnetic Resonance Imaging. Galectin-1 (gal-1), a protein overexpressed in well-differentiated thyroid cancer, has been targeted with a randomized linear 12-mer peptide library using the phage display technique. Selected peptides have been conjugated to ultrasmall superparamagnetic particles of iron oxide (USPIO). Peptides and their corresponding contrast agents have been tested in vitro for their specific binding and toxicity. Two peptides (P1 and P7) were selected according to their affinity towards gal-1. Their binding has been revealed by immunohistochemistry on human thyroid cancer biopsies and they were co-localized with gal-1 by immunofluorescence on TPC-1 cell line. Both peptides induce a decrease of TPC-1 cells’ adhesion to gal-1 immobilized on culture plates. After coupling to USPIO, the peptides preserved their affinity toward gal-1. Their specific binding has been corroborated by co-localization with gal-1 expressed by TPC-1 cells and by their ability to compete with anti-gal-1 antibody. The peptides and their USPIO derivatives produce no toxicity in HepaRG cells as determined by MTT assay. The vectorized contrast agents are potential imaging probes for thyroid cancer diagnosis. Moreover, the two gal-1 targeted peptides prevent cancer cell adhesion by interacting with the carbohydrate recognition domain of gal-1. Keywords: galectin-1, thyroid cancer, peptides, diagnosis, ultrasmall superparamagnetic particles of iron oxide, phage display