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2005-01-01 - Article/Dans un journal avec peer-review - Anglais - 13 page(s)

Saussez Sven , Nonclerq Denis, Laurent Guy , Wattiez Ruddy , André Sabine, Kaltner Herbert, Gabius Hans-Joachim, Kiss Robert, Toubeau Gérard, "Towards functional glycomics by localization of tissue lectins: immunohistochemical galectin fingerprinting during diethylstilbestrol-induced kidney tumorigenesis in male Syrian hamster" in Histochemistry & Cell Biology, 123, 1, 29-41

  • Edition : Springer Science & Business Media B.V., New York (NY)
  • Codes CREF : Biochimie (DI3112), Histologie (DI3212), Biotechnologie (DI3800), Cancérologie (DI3349)
  • Unités de recherche UMONS : Anatomie et Biologie cellulaire (M112), Histologie (M118), Protéomique et Microbiologie (S828)
Texte intégral :

Abstract(s) :

(Anglais) The current study focused on galectins (-1, -3, -4, -7, and -8) and deliberately performed immunohistochemical fingerprinting to explore their complexity in a context of experimental renal carcinogenesis. The diethylstilbestrol (DES)-induced renal tumors in male Syrian hamster kidney (SHKT) represent a unique animal model for the study of estrogen-dependent renal malignancies. Kidney sections of DES-treated hamsters (3 days to 11 months of DES exposure) were analyzed by immunohistochemistry using a panel of non-crossreactive antibodies raised against galectins-1, -3, -4, -7, and -8. Levels of expression were quantitatively determined by using computer-assisted microscopy on immunostained tissue sections. Except for galectin-4, all above mentioned galectins were expressed in kidney tumors. Small clusters of galectin-1-positive, most likely preneoplastic cells at the corticomedullary junction were already evident 1 week after DES administration. Galectin-1 and -3 expression was apparently associated with the first steps of the neoplastic transformation, because small tumorous buds were found to be positive after 1 month of treatment. In contrast, galectins-7 and -8 were detected in large tumors and medium-sized tumors, respectively, thereby indicating an involvement in later stages of DES-induced SHKT. Galectins-1, -3, -7, and -8 were also detected by immunofluorescence staining in the HKT-1097 cell line established from SHKT, thus illustrating the stability of galectin expression in tumor cells. Our data document the presence and differential regulation of galectins in the course of renal tumorigenesis in the model of DES-induced SHKT.

Notes :
  • (Anglais) Lecture en ligne: http://www.springerlink.com/content/dkpax79h1grmtkyp/fulltext.pdf
Identifiants :
  • DOI : 10.1007/s00418-004-0733-6
  • ISSN : 0948-6143
  • PMID : 15609042