DI-UMONS : Dépôt institutionnel de l’université de Mons

Recherche transversale
Rechercher
(titres de publication, de périodique et noms de colloque inclus)
2018-11-26 - Colloque/Présentation - poster - Anglais - 1 page(s)

Jeandriens Jérôme, Schepkens Corentin , Swinnen Johannes, Colet Jean-Marie , "1H-NMR investigation of the effects of glutamine deprivation on human melanoma cell metabolism" in Metabolism in Cancer and Stromal Cells (2nd edition), Leuven, Belgium, 2018

  • Codes CREF : Sciences biomédicales (DI3200)
  • Unités de recherche UMONS : Biologie humaine et Toxicologie (M125)
  • Instituts UMONS : Institut des Sciences et Technologies de la Santé (Santé)

Abstract(s) :

(Anglais) Melanoma is a malignant type of skin cancer that involves extensive metabolic reprogramming. Besides activation of glycolysis, known as the Warburg effect, ‘addiction’ to glutamine is a metabolic hallmark. Here, using an 1H-NMR approach, we studied the effects of glutamine deprivation on the metabolism of malignant melanoma cells. To this end, 451-Lu aggressive human melanoma cells were cultured either without glutamine or in the presence of CB-839, a glutaminase inhibitor. 1H-NMR spectra from both intra- and extracellular compartments were analyzed using a multivariate approach to highlight group separation followed by identification of responsible metabolites. Our results indicate that glutamine depletion attenuates the glycolytic activity and slows down melanoma growth. Interestingly, we also noted a metabolic reprogramming of threonine metabolism. These findings confirm that glutamine metabolism is a potential target for therapy and they extend our insight into the metabolic reprogramming upon glutamine depletion of melanoma.