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2018-07-23 - Colloque/Présentation - communication orale - Anglais - 14 page(s)

Romoaldo Gilson , Hambye Stéphanie , Tagliatti Vanessa , Blankert Bertrand , Colet Jean-Marie , "Influence of psychosocial stress on salivary metabonomic profile" in 11e journées scientifiques du Réseau Francophone de Métabonomique et fluxomique, Liège, Belgique, 2018

  • Codes CREF : Analyse et contrôle pharmaceutique (DI3450), Bases générales de la science et de la culture (DI0100)
  • Unités de recherche UMONS : Métrologie et Sciences du langage (P362), Biologie humaine et Toxicologie (M125), Analyse pharmaceutique (M130)
  • Instituts UMONS : Institut des Sciences et Technologies de la Santé (Santé)
Texte intégral :

Abstract(s) :

(Anglais) Influence of psychosocial stress on salivary metabonomic profile G. Romoaldo1,2, S. Hambye2, V. Tagliatti1, J-M. Colet1, B. Blankert2 1 Laboratory of Human Biology and toxicity, Faculty of Medicine and Pharmacy, UMons 2 Laboratory of Pharmaceutical Analysis, Faculty of Medicine and Pharmacy, UMons, Research Institute for Health Sciences and Technology, Place du Parc 20, 7000 Mons, Belgium. Different biomarkers used for assessing emotional states of individuals are documented in the literature. Most of methods uses plasma as biofluid which collection can occur a stress and requires qualified personnel. Our study aims at monitoring stress, cognitive (over)load and mental fatigue using non-invasive techniques. For this purpose, we used saliva as the biological matrix since its sampling is easy and less likely to induce stress to the subjects. Samples are studied following a double strategy: a targeted approach combined with a non-targeted one. The targeted method concerns analytical quantification of different salivary biomarkers associated to emotional states such as 3-Methoxy-4-hydroxyphenylglycol (MHPG), a norepinephrine metabolite. On the other hand, we studied the evolution of the salivary metabonomic profile. 17 young male students are submitted to a modified version of the Trier social Stress Test (TSST) protocol to induce a psychosocial stress. Saliva samples are collected from subjects and processed using 1HNMR spectra. Evolution of the salivary metabonomic profiles is studied by a multivariate analysis (PCA and PLS-DA) approach and permits to assess the correlation between the fluctuation of emotional states and the variability of metabolites content of this biofluid. Preliminary results showed a variation of the metabonomic profiles after the stress step of the TSST with a back to the initial profile trend following the 30 minutes resting step. Considering these results, based on the NMR spectra, we identify potential biomarkers involved in metabonomic profiles changes due to stress (e.g. mannose, sacharopine, or formate).