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2017-10-01 - Colloque/Présentation - communication orale - Anglais - 1 page(s)

Castro Cristiana , Duprez Marie-Eve , Senechal Tangi , Rhazi Noureddine, Hantson Anne-Lise , "Effect of carbon sources supplementation on formaldehyde-degrading microorganisms growth and formaldehyde dehydrogenase bioproduction and stability" in 10th World Congress of Chemical Engineering, Barcelone, Espagne, 2017

  • Codes CREF : Biochimie (DI3112), Biotechnologie (DI3800), Environnement et pollution (DI3840)
  • Unités de recherche UMONS : Génie des Procédés chimiques et biochimiques (F505)
  • Instituts UMONS : Institut des Biosciences (Biosciences)

Abstract(s) :

(Anglais) Indoor pollution, frequently associated to several illnesses, has attracted attention to the indoor air quality of residential units and workplaces. The most prominent and representative part of indoor pollutants corresponds to volatile organic compounds (VOCs). Hundreds of VOCs, coming from household products, adhesives, building materials, and combustion smoke, can be simultaneously found in mixture with the other contaminants. VOCs are easily absorbed by the skin and mucous membranes, causing damaging consequences to human organs and metabolic systems. So, regulatory pressure on admissible levels of VOCs in indoor air and the emission ranges of the contaminating-materials, is increasing in Europe. Formaldehyde (FA) is one of the most representative oxygenated-VOCs. More than 65 % of global FA is used to synthetize resins, widely used in construction materials, wood processing, furniture, textiles and chemical industries. It is classified as a human carcinogen with adverse effects in health [1], and so, its removal is of widespread interest. Physical, chemical and biological strategies have been developed for cleaning the indoor air from FA. The degradation through biological or enzymatic transformation, is being more and more explored as a flexible, low-cost and efficient FA removal strategy, respecting health and environmental procedure. Formaldehyde dehydrogenase (FDH) and alcohol oxidase (AOX) are two of the enzymes able to use FA as substrate, and transform it to other less toxic compounds, as formic acid and hydrogen peroxide which lead ultimately to carbon dioxide and water. Several microorganisms, including bacteria, cyanobacteria, fungi or yeasts, are known to synthetize FDH and AOX [2,3]. Pseudomonas putida is a natural FDH-producing bacteria, commonly used in for the biodegradation of FA. The adaptation of these cells to specific carbon and energy sources before putting them in contact with FA can induce the over-synthesis of FDH and so, improve the efficiency of the degradation of this polluting agent. It is herein proposed the improvement of FDH synthesis by P. putida strains, in order to optimize the FA degradation through biological transformation. Biomass growth and FDH production and stability were evaluated according to each carbon and energy sources (1%) present in the fermentation culture, namely, glucose, mannitol, sorbitol, glycerol, ethanol, methanol, alanine, trehalose, ribose, xylose and lactose. According to the strategies used, it was possible to increase the total content in proteins, including the FDH, which was confirmed by the SDS-Page assays. In further work, the incorporation of these microorganisms, able to synthetize the depolluting molecules, into the coating will be applied as an innovative solution for indoor FA decontamination. [1] Salthammer T. et al. (2010), Chem. Rev., 110. [2] Roca A. et al. (2008), Microb. Biotechnol., 1,158–169. [3] Fujii T. et al. (1975), Agric. Biol. Chem. 39, 2325–2330.