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2017-11-06 - Article/Dans un journal avec peer-review - Anglais - 5 page(s)

Gossuin Yves , Okusa P.N., Vuong Quoc Lam , Duez Pierre , "NMR relaxation properties of the synthetic malaria pigment beta-hematin" in Scientific Reports, 7, 14557, doi:10.1038/s41598-017-15238-3

  • Edition : Nature Publishing Group, London (United Kingdom)
  • Codes CREF : Résonance magnétique nucléaire (biophysique) (DI131B), Physique du spin (genre RMN) (DI1234), Biophysique (DI3113)
  • Unités de recherche UMONS : Physique biomédicale (M104)
  • Instituts UMONS : Institut des Sciences et Technologies de la Santé (Santé)
  • Centres UMONS : Physique des matériaux (CRPM)
Texte intégral :

Abstract(s) :

(Anglais) 200 million patients sufer from malaria, a parasitic disease caused by protozoans of the genus Plasmodium. Reliable diagnosis is crucial since it allows the early detection of the disease. The development of rapid, sensitive and low-cost diagnosis tools is an important research area. Diferent studies focused on the detection of hemozoin, a major by-product of hemoglobin detoxifcation by the parasite. Hemozoin and its synthetic analog, β-hematin, form paramagnetic crystals. A new detection method of malaria takes advantage of the paramagnetism of hemozoin through the efect that such magnetic crystals have on Nuclear Magnetic Resonance (NMR) relaxation of water protons. Indeed, magnetic microparticles cause a shortening of the relaxation times. In this work, the magnetic properties of two types of β-hematin are assessed at diferent temperatures and magnetic felds. The pure paramagnetism of β-hematin is confrmed. The NMR relaxation of β–hematin suspensions is also studied at diferent magnetic felds and for diferent echo-times. Our results help to identify the best conditions for β–hematin detection by NMR: T2 must be selected, at large magnetic felds and for long echo-times. However, the efect of β-hematin on relaxation does not seem large enough to achieve accurate detection of malaria without any preliminary sample preparation, as microcentrifugation.