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2017-06-15 - Colloque/Présentation - poster - Allemand - 1 page(s)

Beraud Mélanie , Gillan David , Wattiez Ruddy , "Targeted metaproteomic analysis suggests the use of oxidative stress to survive zinc exposure" in 2nd International Metaproteomics Symposium, Alghero, Italie, 2017

  • Codes CREF : Biochimie (DI3112), Biologie moléculaire (DI3111), Biologie (DI3100), Microbiologie et protistologie [bacteriol.,virolog.,mycolog.] (DI3130), Ecologie (DI3123)
  • Unités de recherche UMONS : Protéomie et Microbiologie (S828)
  • Instituts UMONS : Institut des Biosciences (Biosciences)
Texte intégral :

Abstract(s) :

(Anglais) Background Microbial communities are often complex consisting of hundreds of species which can interact with each other by antagonistic or cooperative processes. Understanding how complex microbial communities function and respond to environmental stresses is one of the major challenges of microbiology. In vitro synthetic microbial ecosystems may be designed to reduce the complexity of natural microbial communities. These synthetic microbial consortia, composed of only a few cultivated species, are of growing interest in microbial ecology because they offer a powerful approach whereby biological questions can be addressed in a well-defined framework. Aim The aim of the present research was to analyze the effect of a Zn stress (0.5 mM) on a synthetic microbial community composed of 8 species. Methods A model synthetic marine community was build by the combination of the following 8 species: Pseudomonas putida, Shewanella baltica, Shewanella frigidimarina, Mycobacterium vanbaalenii, Burkholderia xenovorans, Burkholderia glumae, Cupriavidus metallidurans, and Escherichia coli. A Zn stress was then applied and the community was incubated for 28 days at 16°C. The structure of the community was followed by quantitative PCR (Q-PCR) and metaproteomics using the SWATH-MS approach. Results After 28 days, Q-PCR indicated that the synthetic community was composed of very abundant (more than 80 %) and rare (less than 5 %) species. Using the SWATH-MS approach we were able to demonstrate that the Zn treatment induced significant changes in protein levels involved in the treatment of oxydation stress, even for the rare species. Conclusions The SWATH metaproteomic approach is a powerful tool to analyze functionality of rare bacterial species in synthetic bacterial communities. We demonstrated that the first line of defense of our synthetic community against excessive Zn levels are proteins involved in oxidative stress protection.